Analysis of chloroquine and metabolites directly from whole-body animal tissue sections by liquid extraction surface analysis (LESA) and tandem mass spectrometry
Analysis of chloroquine and metabolites directly from whole-body animal tissue sections by liquid extraction surface analysis (LESA) and tandem mass spectrometry
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DOI：
10.1002/jms.3068
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发表时间：
2012-11-01
影响因子：
2.3
通讯作者：
Van Berkel, Gary J.
中科院分区：
文献类型：
--
作者：
Parson, Whitney B.;Koeniger, Stormy L.;Van Berkel, Gary J.
通过液体萃取表面采样分析结合串联质谱（LESA-MS/MS），可以快速、直接地分析组织切片中外源性氯喹（CHQ）和CHQ代谢物的含量和空间分布。LESA-MS/MS结果与先前发表的通过器官切除、提取和荧光检测收集的CHQ定量数据进行了很好的比较。以最少的样品制备和分析方法开发从组织切片直接采样和分析空间分辨的外源性分子的能力具有促进药物化合物的靶组织渗透的评估、建立药代动力学/药效学关系以及补充在组织分布评估期间在药物发现过程中使用的已建立的药代动力学方法的潜力。版权所有（c）2012约翰威利父子有限公司
The rapid and direct analysis of the amount and spatial distribution of exogenous chloroquine (CHQ) and CHQ metabolites from tissue sections by liquid extraction surface sampling analysis coupled with tandem mass spectrometry (LESA-MS/MS) was demonstrated. LESA-MS/MS results compared well with previously published CHQ quantification data collected by organ excision, extraction and fluorescent detection. The ability to directly sample and analyze spatially resolved exogenous molecules from tissue sections with minimal sample preparation and analytical method development has the potential to facilitate the assessment of target tissue penetration of pharmaceutical compounds, to establish pharmacokinetic/pharmacodynamic relationships, and to complement established pharmacokinetic methods used in the drug discovery process during tissue distribution assessment. Copyright (c) 2012 John Wiley & Sons, Ltd.