SECONDARY STRUCTURE MODEL FOR BACTERIAL 16S RIBOSOMAL-RNA - PHYLOGENETIC, ENZYMATIC AND CHEMICAL EVIDENCE
SECONDARY STRUCTURE MODEL FOR BACTERIAL 16S RIBOSOMAL-RNA - PHYLOGENETIC, ENZYMATIC AND CHEMICAL EVIDENCE
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DOI：
10.1093/nar/8.10.2275
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
NOLLER, HF
中科院分区：
文献类型：
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作者：
WOESE, CR;MAGRUM, LJ;NOLLER, HF
我们在比较序列分析、化学修饰研究和核酸酶敏感性数据的基础上推导出了16S核糖体RNA的二级结构模型。theE的核苷酸序列。coliandB。利用100多种真细菌的16S rRNA短链和RNAse t1低聚物目录进行系统发育比较。在裸16S rRNA中，G被乙二醛修饰，A被氯过苯甲酸修饰，C被亚硫酸酯修饰，G被酮醛修饰，在活性和非活性30S核糖体亚基中作为单链结构的指示。对rna酶A和T1的敏感性进一步支持了该结构。这三种方法非常一致。该结构包含50个螺旋元件，分为四个主要结构域，其中46%的16S rRNA核苷酸参与碱基配对。系统发育比较表明，高度保守的序列主要存在于分子的非配对区域。这种结构不会产生结。
We have derived a secondary structure model for 16S ribosomal RNA on the basis of comparative sequence analysis, chemical modification studies and nuclease susceptibility data. Nucleotide sequences of theE. coliandB. brevis16S rRNA chains, and of RNAse T1oligomer catalogs from 16S rRNAs of over 100 species of eubacteria were used for phylogenetic comparison. Chemical modification of G by glyoxal, A bym-chloroperbenzoic acid and C by bisulfite In naked 16S rRNA, and G by kethoxal in active and inactive 30S ribosomal subunits was taken as an indication of single stranded structure. Further support for the structure was obtained from susceptibility to RNases A and T1. These three approaches are in excellent agreement. The structure contains fifty helical elements organized into four major domains, in which 46 percent of the nucleotides of 16S rRNA are involved in base pairing. Phylogenetic comparison shows that highly conserved sequences are found principally in unpaired regions of the molecule. No knots are created by the structure.