Transmission of Rickettsia tsutsugamushi strains among humans, wild rodents, and trombiculid mites in an area of Japan in which tsutsugamushi disease is newly endemic
Transmission of Rickettsia tsutsugamushi strains among humans, wild rodents, and trombiculid mites in an area of Japan in which tsutsugamushi disease is newly endemic
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在日本恙虫病新流行地区，恙虫病立克次体菌株在人类、野生啮齿动物和螨类中的传播
DOI：
10.1128/jcm.32.11.2780-2785.1994
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发表时间：
1994
影响因子：
9.4
通讯作者：
J. S. Kang
中科院分区：
文献类型：
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作者：
T. Yamashita;S. Kasuya;N. Noda;I. Nagano;J. S. Kang
用间接荧光抗体试验检测了32株新分离的恙虫病立克次体（14株来自恙虫病患者，12株来自野生啮齿动物，6株来自寄生于日本岐阜县啮齿动物的恙螨）对12种单克隆抗体的反应性，并对其抗原性进行了分类。根据对单克隆抗体的反应性，所有分离株可分为KN-1、KN-2、KN-3、GJ-1、R158和R161六个群。在岐阜县患者中流行的KN-1和GJ-1菌株分别与在日本西南部宫崎县和鹿儿岛县分离并流行的川崎和黑木菌株具有相同的反应性。患者分离株与啮齿类和螨类分离株血清型不同。KN-2和KN-3菌株分别在患者、啮齿动物和螨类中最流行。KN-2与其它供试菌株之间没有观察到相似性。KN-3仅是岐阜县患者疾病的次要贡献者;然而，已证明相同菌株在日本北方的新泻县流行。因此，岐阜县是南、北方和地方株的分布地区，我们推测人类容易感染KN-2、GJ-1（与黑木非常相似）和KN-1（与川崎非常相似），可能是由于感染了小盾纤恙螨。而L. scutellare和L.梅毒螺旋体寄生于野生啮齿类动物，可携带任何立克次体菌株，最强毒力菌株KN-3在野生啮齿类动物中占优势。用抗R.恙虫病应是有用的流行病学研究感染这种生物体。
Thirty-two newly isolated strains of Rickettsia tsutsugamushi, 14 from patients with tsutsugamushi disease, 12 from wild rodents, and 6 from trombiculid mites parasitizing rodents in Gifu Prefecture, Japan, were examined for reactivities to 12 monoclonal antibodies by an indirect fluorescent-antibody test to classify their antigenicities. All of the isolates could be classified into one of six groups (KN-1, KN-2, KN-3, GJ-1, R158, and R161) according to their reactivities to the monoclonal antibodies. The KN-1 and GJ-1 strains that are prevalent among patients from Gifu Prefecture had the same reactivities as the Kawasaki and Kuroki strains, respectively, which have been isolated and are prevalent in the Miyazaki and Kagoshima prefectures in southwest Japan. The isolates from patients were different in serotype from those from rodents and mites (Leptotrombidium pallidum). The KN-2 and KN-3 strains were most prevalent among patients and among rodents and mites, respectively. No close similarity between KN-2 and other strains tested was observed. KN-3 is only a minor contributor to diseases in patients in Gifu Prefecture; however, it was proven that the same strain was prevalent in Niigata Prefecture in northern Japan. Thus, Gifu Prefecture is an area where southern, northern, and local strains are found. We hypothesize that humans are prone to infection with KN-2, GJ-1 (very similar to Kuroki), and KN-1 (very similar to Kawasaki), probably by infestation with Leptotrombidium scutellare. While both L. scutellare and L. pallidum parasitize wild rodents and may carry any rickettsial strain, the most virulent strain, KN-3, is predominant among wild rodents. Antigenic analysis using monoclonal antibodies to R. tsutsugamushi should be useful for epidemiological studies of infection with this organism.
Kawamori, Ohashi, N. 和 Tamura, A.等人：“恙虫病的流行病学与从日本静冈县富士山东坡的患者、田鼠和未喂食的恙虫中分离出的恙虫病立克次体血清型有关
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Ohashi，Norio：“从日本患者体内分离出恙虫病立克次体的新抗原类型黑木株的特征”
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