Bioequivalence of docosahexaenoic acid and α-linolenic acid supplementations on plasmalogen, long-chain aldehyde, and docosahexaenoic acid levels in the brain of very old rats
Bioequivalence of docosahexaenoic acid and α-linolenic acid supplementations on plasmalogen, long-chain aldehyde, and docosahexaenoic acid levels in the brain of very old rats
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DOI：
10.1016/j.nutres.2006.04.006
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发表时间：
2006-05-01
影响因子：
4.5
通讯作者：
Chardigny, Jean-Michel
中科院分区：
文献类型：
--
作者：
Andre, Agnes;Cabaret, Stephanie;Chardigny, Jean-Michel
老化的大脑在其脂质成分，特别是血浆蛋白原(P1)水平上经历了改变。这些磷脂是已知的多不饱和脂肪酸的储存库，通常被认为是内源性抗氧化剂。本研究的目的是量化21个月龄大鼠大脑中的P1和游离醛(P1氧化产物)，并确定饮食中α-亚麻酸(ALA)和二十二碳六烯酸(DHA)对这些水平的影响。来自两代n-3缺陷型动物的大鼠(21个月)分别饲喂n-3脂肪酸缺乏的饲料--仅含ALA的饲料或添加0.6%DHA的饲料。在21个月和饮食3个月或5个月后，评估海马体、皮质和小脑中的P1、游离醛和DHA水平。24月龄n-3缺陷大鼠大脑皮质和小脑中的纤溶酶原水平显著降低。营养水平的ALA和DHA对老年大鼠脑内DHA水平的恢复效果相同。日粮n-3脂肪酸对血浆蛋白原和游离醛水平无显著影响。与n-3脂肪酸缺乏的情况相比，富含DHA的磷脂对血浆原的氧化作用并不明显。总而言之，在衰老过程中，大脑某些结构中的P1水平会下降。乙醛水平的变化可能无法解释生理老化过程中发生的变化。最后，膳食ALA和DHA作为二十二碳六烯酸积累来源的生物等效性在老年大鼠脑中可能是相似的。(C)2006 Elsevier Inc.保留所有权利。
The aging brain undergoes modifications in its lipid composition and, especially, in plasmalogen (P1) levels. These phospholipids are known to be reservoirs of polyunsaturated fatty acids and often considered as endogenous antioxidants. The objectives of this study were to quantify P1s and free aldehydes (products of P1 oxidation) in the rat brain after 21 months of age and to determine the impact of dietary alpha-linolenic acid (ALA) and docosahexaenoic acid (DHA) on these levels. Rats (21 months) from 2 generations of n-3-deficient animals were fed either a n-3 fatty acid-deficient diet-a diet containing ALA alone or with 0.6% of DHA. At 21 months and after 3 or 5 months of diet, P1, free aldehyde, and DHA levels were assessed in the hippocampus, cortex, and cerebellum. Plasmalogen levels significantly decreased in the cortex and cerebellum of n-3-deficient 24-month-old rats. Dietary ALA and DHA at nutritional levels have the same efficiency to restore DHA levels in the brain of aged rat. Plasmalogen and free aldehyde levels were not significantly influenced by dietary n-3 fatty acids. Plasmalogens were not more oxidized when phospholipids were enriched in DHA, compared with the situation of n-3 fatty acid deficiency. In conclusion, during aging, P1 levels decrease in some structures of the brain. The evolution of aldehyde levels may not explain changes occurring during physiologic aging. Lastly, the bioequivalence of dietary ALA and DHA as sources of docosahexaenoate accretion could be similar in aged rat brain. (c) 2006 Elsevier Inc. All rights reserved.