FEEDING-BEHAVIOR AND SPOROZOITE EJECTION BY INFECTED ANOPHELES-STEPHENSI
FEEDING-BEHAVIOR AND SPOROZOITE EJECTION BY INFECTED ANOPHELES-STEPHENSI
复制标题
DOI：
10.1016/0035-9203(91)90012-n
复制
发表时间：
1991-03-01
影响因子：
2.2
通讯作者：
MEUWISSEN, JHET
中科院分区：
文献类型：
--
作者：
PONNUDURAI, T;LENSEN, AHW;MEUWISSEN, JHET
允许感染恶性疟原虫子孢子的史氏按蚊通过新鲜的全层小鼠皮肤单独进食。成簇射入皮肤的子孢子数量多于射入血液的子孢子数量。子孢子在血液中的沉积很少发生，并且总是与这些阶段喷射到皮肤中同时发生——这可能是溢出效应。喂食前的探针数量（中位数 4.5）与子孢子接种量（中位数 8）不相关，腺体中的子孢子数量（中位数 14 500）也不相关。然而，皮肤中子孢子簇的数量（中位数1）与接种量呈正相关。当仅包括那些喷射子孢子的蚊子时，子孢子接种量的中值为 22。当中断喂食并建议使用新膜时，两种情况下都会以相同的频率发生子孢子排出。活体小鼠在喂食后 2 小时内，子孢子就从被咬伤处消失。讨论了这些观察结果的流行病学意义。
Anopheles stephensi mosquitoes infected with Plasmodium falciparum sporozoites were allowed to feed individually through fresh whole thickness mouse skin. More sporozoites were ejected into the skin in clusters than into the blood. Deposition of sporozoites in the blood was an infrequent occurrence and always coincided with ejection of these stages into the skin-perhaps a spill-over effect. The number of probes before feeding (median 4.5) was not correlated with the sporozoite inoculum (median 8), nor was the number of sporozoites in the glands (median 14 500). However, the number of sporozoite clusters in the skin (median 1) was positively correlated with the inoculum size. The median value of the sporozoite inoculum was 22, when only those mosquitoes that ejected sporozoites were included. When feeding was interrupted and recommended on a new membrane, sporozoite ejection occurred with equal frequency on both occasions. Sporozoites disappeared from the site of bites in living mice within 2 h of feeding. The epidemiological significance of these observations is discussed.