Identification and Characterization of an Arginine Kinase as a Major Allergen from Silkworm (Bombyx mori) Larvae
Identification and Characterization of an Arginine Kinase as a Major Allergen from Silkworm (Bombyx mori) Larvae
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DOI：
10.1159/000210375
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Roux, Kenneth H.
中科院分区：
文献类型：
--
作者：
Liu, Zhigang;Xia, Lixin;Roux, Kenneth H.
背景：蚕（Bombyx mori）是纺织工业中的重要昆虫，其蛹用于中国烹饪和中药。蚕丝、尿液和皮屑常常是养蚕工人过敏的原因，而蚕蛹在中国已被发现是食物过敏原。最近的研究重点是报告蚕过敏病例，但只有少数研究涉及家蚕中存在的特定过敏原。方法：我们收集了 10 名蚕粗提物 (SCE) 皮肤点刺试验阳性患者的血清，并通过蛋白质印迹和 ELISA 分析了这些样本。来自家蚕的精氨酸激酶的cDNA也被克隆并在大肠杆菌中高产表达。通过 ELISA 抑制试验研究了重组家蚕精氨酸激酶 (rBmAK) 的过敏原性和交叉过敏性。结果：以SCE为抗原的Western印迹中，收集的血清均对42-kDa蛋白发生反应。血清与 rBmAK 预孵育消除了患者血清对该 42 kDa 条带的反应性。在 ELISA 测定中，所有患者血清也表现出对 SCE 的阳性反应性。 BmAK 还表现出与蟑螂重组 AK 的交叉反应性。结论：来自家蚕的精氨酸激酶是一种主要过敏原，并与蟑螂 AK 发生交叉反应。版权所有 (C) 2009 S. Karger AG，巴塞尔
Background: The silkworm, Bombyx mori, is an important insect in the textile industry and its pupa are used in Chinese cuisine and traditional Chinese medicine. The silk, urine and dander of silkworms is often the cause of allergies in sericulture workers and the pupa has been found to be a food allergen in China. Recent studies have focused on reporting cases of silkworm allergies, but only a few studies have addressed the specific allergens present in the B. mori silkworm. Methods: We collected sera from 10 patients with a positive skin prick test to silkworm crude extract (SCE) and analyzed these samples by Western blot and ELISA. The cDNA of arginine kinase from the B. mori silkworm was also cloned and expressed in high yield in Escherichia coli. Allergenicity and cross-allergenicity of the recombinant B. mori arginine kinase (rBmAK) were investigated by ELISA inhibition assay. Results: Collected sera all reacted to a 42-kDa protein in a Western blot with SCE as the antigen. Preincubation of sera with rBmAK eliminated the reactivity of the patients' sera to this 42-kDa band. All patient sera also exhibited positive reactivity to SCE in an ELISA assay. BmAK also demonstrated cross-reactivity with a recombinant AK from cockroach. Conclusion: Arginine kinase from the B. mori silkworm is a major allergen and crossreacts with cockroach AK. Copyright (C) 2009 S. Karger AG, Basel