The Composition of Human Milk and Infant Faecal Microbiota Over the First Three Months of Life: A Pilot Study.
The Composition of Human Milk and Infant Faecal Microbiota Over the First Three Months of Life: A Pilot Study.
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DOI：
10.1038/srep40597
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发表时间：
2017-01-17
影响因子：
4.6
通讯作者：
Stanton C
中科院分区：
文献类型：
--
作者：
Murphy K;Curley D;O'Callaghan TF;O'Shea CA;Dempsey EM;O'Toole PW;Ross RP;Ryan CA;Stanton C
母乳含有多种生物活性物质，也是婴儿肠道发育的细菌来源。本研究的目的是描述10对母婴在出生后前3个月内母乳和婴儿粪便中的细菌群落。还评估了人乳中活双歧杆菌和乳杆菌的存在。MiSeq测序揭示了母乳微生物群的巨大多样性，在牛奶样品中鉴定了超过207种细菌属。变形菌门和厚壁菌门以及假单胞菌属、葡萄球菌属和链球菌属是主要的细菌类群。在第1、3和6周，12个属的核心代表了牛奶样品中微生物群相对丰度的81%，在第12周下降到73%。婴儿粪便和人乳样品之间共有的属占婴儿粪便样品中总相对丰度的70-88%，支持细菌从乳汁垂直转移到婴儿肠道的假设。此外，从一对母婴的乳汁和粪便中分离出相同的短双歧杆菌和植物乳杆菌菌株。通过母乳喂养的细菌垂直转移可能有助于在发育中的婴儿肠道中初步建立微生物群。
Human milk contains a diverse array of bioactives and is also a source of bacteria for the developing infant gut. The aim of this study was to characterize the bacterial communities in human milk and infant faeces over the first 3 months of life, in 10 mother-infant pairs. The presence of viable Bifidobacterium and Lactobacillus in human milk was also evaluated. MiSeq sequencing revealed a large diversity of the human milk microbiota, identifying over 207 bacterial genera in milk samples. The phyla Proteobacteria and Firmicutes and the genera Pseudomonas, Staphylococcus and Streptococcus were the predominant bacterial groups. A core of 12 genera represented 81% of the microbiota relative abundance in milk samples at week 1, 3 and 6, decreasing to 73% at week 12. Genera shared between infant faeces and human milk samples accounted for 70–88% of the total relative abundance in infant faecal samples, supporting the hypothesis of vertical transfer of bacteria from milk to the infant gut. In addition, identical strains of Bifidobacterium breve and Lactobacillus plantarum were isolated from the milk and faeces of one mother-infant pair. Vertical transfer of bacteria via breastfeeding may contribute to the initial establishment of the microbiota in the developing infant intestine.