Accuracy, limitations and cost efficiency of eDNA-based community survey in tropical frogs
Accuracy, limitations and cost efficiency of eDNA-based community survey in tropical frogs
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DOI：
10.1111/1755-0998.12934
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发表时间：
2018-11-01
影响因子：
7.7
通讯作者：
Jansen, Martin
中科院分区：
文献类型：
--
作者：
Balint, Miklos;Nowak, Carsten;Jansen, Martin
在生物多样性高度丰富的热带地区，快速的环境变化需要有效的生物监测方案。虽然现有的物种多样性和群落组成指标依赖于基于遭遇的调查数据，但eDNA最近成为一种替代方法。基于edna的方法在热带地区的成本和生态价值很少得到评估，在热带地区，高物种丰富度伴随着高功能多样性（例如，不同物种和生命阶段使用不同的微生境）。我们首先测试了eDNA数据对热带青蛙群落结构的估计是否与专家实地评估相一致。接下来，我们评估了eDNA是否是热带地区生物多样性监测的经济可行的解决方案。本文应用eDNA元条码技术对玻利维亚奇基塔诺干旱林区5个池塘蛙类的发生情况进行了调查，并与同步视觉和音频遭遇调查（VAES）进行了比较。我们发现，eDNA和VAES生成的分类单元列表和群落结构非常接近，大多数偏差可归因于不同物种的生活史。eDNA调查的成本效率主要受当地动物丰富度和调查地点数量的影响：在低多样性地区，VAES可能成本较低，但在高多样性地区，eDNA很快变得更具成本效益。结果表明，如果考虑生命史，eDNA适合于高多样性地区的大规模生物多样性调查，并在采样、遗传分析和数据解释中采取一定的注意事项。我们预计，空间上广泛的、标准化的eDNA生物多样性调查将在未来迅速出现。
Rapid environmental change in highly biodiverse tropical regions demands efficient biomonitoring programmes. While existing metrics of species diversity and community composition rely on encounter-based survey data, eDNA recently emerged as alternative approach. Costs and ecological value of eDNA-based methods have rarely been evaluated in tropical regions, where high species richness is accompanied by high functional diversity (e.g., the use of different microhabitats by different species and life stages). We first tested whether estimation of tropical frogs' community structure derived from eDNA data is compatible with expert field assessments. Next, we evaluated whether eDNA is a financially viable solution for biodiversity monitoring in tropical regions. We applied eDNA metabarcoding to investigate frog species occurrence in five ponds in the Chiquitano dry forest region in Bolivia and compared our data with a simultaneous visual and audio encounter survey (VAES). We found that taxon lists and community structure generated with eDNA and VAES correspond closely, and most deviations are attributable to different species' life histories. Cost efficiency of eDNA surveys was mostly influenced by the richness of local fauna and the number of surveyed sites: VAES may be less costly in low-diversity regions, but eDNA quickly becomes more cost-efficient in high-diversity regions with many sites sampled. The results highlight that eDNA is suitable for large-scale biodiversity surveys in high-diversity areas if life history is considered, and certain precautions in sampling, genetic analyses and data interpretation are taken. We anticipate that spatially extensive, standardized eDNA biodiversity surveys will quickly emerge in the future.