Ionic liquid matrix for direct UV-MALDI-TOF-MS analysis of dermatan sulfate and chondroitin sulfate oligosaccharides
Ionic liquid matrix for direct UV-MALDI-TOF-MS analysis of dermatan sulfate and chondroitin sulfate oligosaccharides
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DOI：
10.1021/ac061688m
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发表时间：
2007-02-15
影响因子：
7.4
通讯作者：
Linhardt, Robert J.
中科院分区：
文献类型：
--
作者：
Laremore, Tatiana N.;Zhang, Fuming;Linhardt, Robert J.
聚阴离子寡糖如硫酸皮肤聚糖（DS）和硫酸软骨素（CS）表现出较差的电离效率，并且在常规紫外基质辅助激光解吸/电离（UV-MALDI）条件下容易因SO3的损失而发生热碎裂。一种新的离子液体基质（ILM），一种α -氰基-4-羟基肉桂酸的胍盐，有助于直接紫外- maldi质谱（MS）分析未酸化的DS和CS低聚糖，其通常形式为钠盐。由此产生的质谱显示，由于SO3的损失，碎片化程度很低。新ILM适用于含有不同亚砜基团的低聚糖混合物的MALDI-MS分析。
Polyanionic oligosaccharides such as dermatan sulfate (DS) and chondroitin sulfate (CS) exhibit poor ionization efficiencies and tend to undergo thermal fragmentation through the loss of SO3 under conventional ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI) conditions. A new ionic liquid matrix (ILM), a guanidinium salt of alpha-cyano-4-hydroxycinnamic acid, facilitates direct UV-MALDI mass spectrometric (MS) analysis of underivatized DS and CS oligosaccharides up to a decasaccharide in their common form as sodium salts. The resulting mass spectra show very low extent of fragmentation through an SO3 loss. The new ILM is suitable for MALDI-MS analysis of mixtures containing oligosaccharides with different numbers of sulfo groups.