Metalloproteinase 9 is the outer executioner of desmoglein 3 in apoptotic keratinocytes
Metalloproteinase 9 is the outer executioner of desmoglein 3 in apoptotic keratinocytes
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DOI：
10.1111/j.1601-0825.2006.01287.x
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发表时间：
2007-05-01
期刊：
影响因子：
3.8
通讯作者：
Lanza, A.
中科院分区：
文献类型：
--
作者：
Cirillo, N.;Femiano, F.;Lanza, A.
目的：探讨凋亡角质形成细胞中特异性靶向desmoglin 3 （Dsg3）的基质金属蛋白酶（MMPs）。方法：对体外培养的角质形成细胞进行抑制实验和Western blot分析。结果：阻断MMP-9活性可显著减少Dsg3从细胞表面的脱落。MMP-2在Dsg3的切割中作用较小，而其他MMPs，如MMP-1、-3和-8不靶向Dsg3。结论：凋亡性角质形成细胞通过MMP-9活性损害细胞表面Dsg3的胞外结构域。发现Dsg3的特异性靶点可能有助于了解Dsg3受影响的疾病的病理生理学。
Objective: To investigate the specific matrix metalloproteinases (MMPs) targeting desmoglein 3 (Dsg3) in apoptotic keratinocytes.Method: Inhibitor studies on cultured keratinocytes and Western blot analysis.Results: Blocking of MMP-9 activity strongly reduces shedding of Dsg3 from cell surface. MMP-2 has a less relevant role in the cleavage of Dsg3 while other MMPs, such as MMP-1, -3, and -8, do not target Dsg3.Conclusions: Apoptic keratinocytes impair the extracellular domain of cell surface Dsg3 by MMP-9 activity. The discovery of a specific targeting of Dsg3 could be useful to understand the pathophysiology of diseases in which Dsg3 is affected.