TN7 TRANSPOSITION - RECOGNITION OF THE ATTTN7 TARGET SEQUENCE
TN7 TRANSPOSITION - RECOGNITION OF THE ATTTN7 TARGET SEQUENCE
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DOI：
10.1073/pnas.86.11.3958
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发表时间：
1989-06-01
影响因子：
11.1
通讯作者：
CRAIG, NL
中科院分区：
文献类型：
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作者：
WADDELL, CS;CRAIG, NL
细菌转座子Tn7编码两条截然不同但重叠的转座途径。TnsABC+tnsD促进转座到一个特定的位点attTn7，而tnsABC+tnsE促进转座到许多其他与attTn7无关的位点。我们已经鉴定出一种依赖tnsD的DNA结合活性，它能特异性地识别attTn7。我们已经将该活性的识别序列定位到一个28个碱基对的区域，并表明这个相同的区域可以在体内提供attTn7靶标的特定性质。有趣的是，这些序列位于距Tn7插入特定点超过25个碱基对的位置。
The bacterial transposon Tn7 encodes two distinct but overlapping transposition pathways. tnsABC + tnsD promote transposition to a specific site, attTn7, while tnsABC + tnsE promote transposition to many other sites unrelated to attTn7. We have identified a tnsD-dependent DNA binding activity that specifically recognizes attTn7. We have localized the recognition sequences for this activity to a 28-base-pair region and have shown that this same region can provide specific properties of an attTn7 target in vivo. Interestingly, these sequences are positioned more than 25 base pairs from the specific point of Tn7 insertion.