STIMULATION OF GOBLET CELL MUCOUS SECRETION BY ACTIVATION OF NERVES IN RAT CONJUNCTIVA
STIMULATION OF GOBLET CELL MUCOUS SECRETION BY ACTIVATION OF NERVES IN RAT CONJUNCTIVA
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DOI：
10.3109/02713689508998519
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发表时间：
1995-11-01
影响因子：
2
通讯作者：
DARTT, DA
中科院分区：
文献类型：
--
作者：
KESSLER, TL;MERCER, HJ;DARTT, DA
上皮清创伤口作为对角膜的刺激，导致该眼结膜杯状细胞分泌粘液。为了确定这种分泌刺激是否是神经介导的，将大鼠麻醉，局部麻醉剂利多卡因（1%）或缓冲液单独局部和/或结膜下给药15分钟。在一只眼睛中进行角膜上皮清创伤口。对侧眼作为对照。5-120分钟后，处死动物，取下下球结膜纽扣。杯状细胞中的粘液用阿尔新蓝和高碘酸-希夫试剂染色以指示含粘蛋白的杯状细胞。通过光学显微镜测定每0.16 mm（2）含粘蛋白杯状细胞的数量;数量减少表明粘液分泌增加。角膜损伤刺激诱导该眼杯状细胞分泌粘液。刺激后5分钟内迅速观察到分泌，长达120分钟。局部应用利多卡因，结膜下注射利多卡因，或两者的组合抑制伤口诱导的刺激粘液分泌。我们的结论是结膜杯状细胞粘液分泌可以神经介导的，可以作为一个即时的反应，以保护眼表。
An epithelial debridement wound, as a stimulus to the cornea, causes conjunctival goblet cell mucous secretion in that eye. To determine if this stimulation of secretion is neurally mediated, rats were anesthetized and the local anesthetic lidocaine (1%) or buffer alone was administered topically and/or subconjunctivally for 15 min. A corneal epithelial debridement wound was made in one eye. The contralateral eye served as the control. After 5-120 min, animals were sacrificed and inferior bulbar conjunctival buttons removed. Mucus in the goblet cells was stained with Alcian blue and periodic acid-Schiff's reagent to indicate mucin-containing goblet cells. The number of mucin-containing goblet cells/0.16 mm(2) was determined by light microscopy; a decrease in number indicated an increase in mucous secretion. Stimulation by corneal wounding induced goblet cell mucous secretion in that eye. Secretion was observed as rapidly as 5 min after stimulus and for as long as 120 min. Topical application of lidocaine, subconjunctival injection of lidocaine, or a combination of both inhibited wound-induced stimulation of mucous secretion. We conclude that conjunctival goblet cell mucous secretion can be neurally mediated and could serve as an immediate response to protect the ocular surface.