Gene microarray analysis of human brain arteriovenous malformations
Gene microarray analysis of human brain arteriovenous malformations
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DOI：
10.1227/01.neu.0000103421.35266.71
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发表时间：
2004-02-01
期刊：
影响因子：
4.8
通讯作者：
Young, WL
中科院分区：
文献类型：
--
作者：
Hashimoto, T;Lawton, MT;Young, WL
目的：人脑。动静脉畸形（BAVM）显示各种血管生成相关基因及其产物的异常表达。我们通过基因微阵列技术检测BAVM中的基因表达模式。方法：我们通过Affyandroid Human Gene Set U95 Av 2（Affyandroid，Inc.，Santa Clara，CA）。基因微阵列数据进行了比较，新的和以前发表的数据，使用传统的分子生物学techniques.RESULTS：我们分析了六个BAVM和五个对照大脑样本。在12,625个基因探针中，1781个基因探针在BAVM和对照之间显示差异表达。BAVM样本的基因表达模式与对照脑样本的基因表达模式不同。血管内皮生长因子A的信使核糖核酸表达增加伴随着其蛋白产物表达的增加。大多数基因数据与先前发表的数据一致。基因芯片数据产生了一个新的可检验的假说，关于整合素，我们发现增加的整合素α v β 3蛋白的表达在BAVMs.CONCLUSION：BAVMs的基因表达模式是不同的控制脑样品。我们证实了基因微阵列数据，证明了血管生成相关分子的基因表达水平的增加伴随着其蛋白质产物表达水平的增加。基因芯片技术可能是一个有用的工具，同时研究多个途径在BAVM标本。
OBJECTIVE: Human brain. arteriovenous malformations (BAVMs) display abnormal expression of various angiogenesis-related genes and their products. We examined gene expression patterns in BAVMs by the gene microarray technique.METHODS: We analyzed BAVM and control brain samples obtained by temporal lobectomy for medically intractable seizure by Affymetrix Human Gene Set U95Av2 (Affymetrix, Inc., Santa Clara, CA). The gene microarray data were compared with new and previously published data that used conventional molecular biology techniques.RESULTS: We analyzed six BAVM and five control brain samples. From 12,625 gene probes assayed, 1781 gene probes showed differential expression between BAVMs and controls. BAVM samples had a gene expression pattern that was distinct from those of control brain samples. Increased messenger ribonucleic acid expression of vascular endothelial growth factor A was accompanied by increased expression of its protein product. A majority of the gene data was in agreement with previously published data. The gene microarray data generated a new testable hypothesis regarding integrin, and we found increased expression of integrin alphavbeta3 protein in BAVMS.CONCLUSION: The gene expression pattern of BAVMs was distinct from those of control brain samples. We verified the gene microarray data by demonstrating that increased gene expression levels for angiogenesis-related molecules were accompanied by increased levels of their protein product expression. The gene microarray technique may be a useful tool to study multiple pathways simultaneously in BAVM specimens.