Good performance of rapid prostate-specific antigen test for detection of semen exposure in women: implications for qualitative research.
Good performance of rapid prostate-specific antigen test for detection of semen exposure in women: implications for qualitative research.
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DOI：
10.1097/olq.0b013e3181a2b4bf
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发表时间：
2009-08
影响因子：
3.1
通讯作者：
Macaluso M
中科院分区：
文献类型：
--
作者：
Hobbs MM;Steiner MJ;Rich KD;Gallo MF;Alam A;Rahman M;Menezes P;Chipato T;Warner L;Macaluso M
前列腺特异性抗原（PSA）是女性精液暴露的有效生物标志物，已用于评估自我报告的性行为的可靠性以及作为安全套功效的替代指标。定量 PSA 测试价格昂贵，并且需要专门的设备。简单、快速且廉价的 PSA 测试将有助于在各种研究环境中评估精液生物标志物。本研究评估了快速 PSA 检测与定量检测相比的性能，以识别阴道拭子样本中的精液。我们测试了从参与孟加拉国和津巴布韦两项独立研究的 492 名女性收集的 581 份阴道拭子。使用定量 IMx (Abbott Laboratories) 测定和 ABAcard p30 (Abacus Diagnostics) 快速免疫层析试纸条测试检测阴道分泌物中的 PSA。与检测 >1.0 ng PSA/mL 阴道拭子洗脱液的定量测试相比，ABAcard 测试的敏感性为 100%（95% 置信区间 [CI]，98%–100%），特异性为 96%（95% CI，93%–97%）。快速 PSA 结果是半定量的，与 PSA 浓度密切相关（κ = 0.88；95% CI，0.85-0.90）。快速 PSA 检测不需要仪器，可以轻松、经济地进行。面谈后立即获得快速 PSA 结果提供了探索近期精液暴露的客观标记与自我报告行为之间差异的机会。
Prostate-specific antigen (PSA) is a valid biomarker of semen exposure in women and has been used to assess reliability of self-reported sexual behavior as well as serve as a proxy measure for condom efficacy. Quantitative PSA tests are expensive and require specialized equipment. A simple, rapid, and inexpensive test for PSA would facilitate semen biomarker evaluation in a variety of research settings. This study evaluated the performance of a rapid PSA test compared with a quantitative assay to identify semen in vaginal swab specimens. We tested 581 vaginal swabs collected from 492 women participating in 2 separate research studies in Bangladesh and Zimbabwe. PSA in vaginal secretions was detected using the quantitative IMx (Abbott Laboratories) assay and the ABAcard p30 (Abacus Diagnostics) rapid immunochromatographic strip test. The ABAcard test was 100% sensitive (95% confidence interval [CI], 98%–100%) and 96% specific (95% CI, 93%–97%) compared with the quantitative test in detecting >1.0 ng PSA/mL vaginal swab eluate. Rapid PSA results were semiquantitative and correlated well with PSA concentrations (κ = 0.88; 95% CI, 0.85–0.90). Rapid PSA detection requires no instrumentation and can be performed easily and economically. Having rapid PSA results available immediately following interview provides opportunities to explore discrepancies between the objective marker of recent semen exposure and self-reported behaviors.