Methodology for determining major constituents of ayahuasca and their metabolites in blood
Methodology for determining major constituents of ayahuasca and their metabolites in blood
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DOI：
10.1002/bmc.1657
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发表时间：
2012-03-01
影响因子：
1.8
通讯作者：
Barker, Steven A.
中科院分区：
文献类型：
--
作者：
McIlhenny, Ethan H.;Riba, Jordi;Barker, Steven A.
死藤水是一种南美精神药物植物茶，在土著医疗和宗教用途方面有着悠久的文化历史，人们对其潜在的医疗应用越来越感兴趣。对死藤水的临床研究需要具体、灵敏和全面的方法来表征和定量这些化合物及其在血液中的代谢物。结合两种分析技术（紫外和/或荧光检测的高效液相色谱法和氮磷检测的气相色谱法）已被用于分析口服死藤水后血液中的一些成分。我们在此报告了一种直接分析死藤水14种主要生物碱成分的单一方法，包括血液中N，N-二甲基色胺和哈玛拉生物碱的几种已知和潜在代谢物。该方法对血浆样品采用96孔板/蛋白沉淀/过滤，并采用加热电喷雾电离的HPLCion捕获质谱法进行分析，以减少基质效应。该方法扩大了在死藤水给药后血液中能够监测的化合物清单，同时提供了一种简化的分析方法。该方法具有良好的灵敏度、特异性和重复性，可用于死藤水的临床研究。版权所有：John Wiley & Sons， Ltd。
There is an increasing interest in potential medical applications of ayahuasca, a South American psychotropic plant tea with a long cultural history of indigenous medical and religious use. Clinical research into ayahuasca will require specific, sensitive and comprehensive methods for the characterization and quantitation of these compounds and their metabolites in blood. A combination of two analytical techniques (high-performance liquid chromatography with ultraviolet and/or fluorescence detection and gas chromatography with nitrogenphosphorus detection) has been used for the analysis of some of the constituents of ayahuasca in blood following its oral consumption. We report here a single methodology for the direct analysis of 14 of the major alkaloid components of ayahuasca, including several known and potential metabolites of N,N-dimethyltryptamine and the harmala alkaloids in blood. The method uses 96-well plate/protein precipitation/filtration for plasma samples, and analysis by HPLCion trapion trapmass spectrometry using heated electrospray ionization to reduce matrix effects. The method expands the list of compounds capable of being monitored in blood following ayahuasca administration while providing a simplified approach to their analysis. The method has adequate sensitivity, specificity and reproducibility to make it useful for clinical research with ayahuasca. Copyright (C) 2011 John Wiley & Sons, Ltd.