Preparation and anticoagulant properties of heparin-like electrospun membranes from carboxymethyl chitosan and bacterial cellulose sulfate
Preparation and anticoagulant properties of heparin-like electrospun membranes from carboxymethyl chitosan and bacterial cellulose sulfate
复制标题
羧甲基壳聚糖和硫酸细菌纤维素类肝素电纺膜的制备及其抗凝性能
DOI：
10.1016/j.ijbiomac.2018.09.133
复制
发表时间：
2018
影响因子：
8.2
通讯作者：
Changjiang Pan
中科院分区：
文献类型：
--
作者：
Weikang Song;Qinhuan Zeng;Xueqiong Yin;Li Zhu;Tao Gong;Changjiang Pan
将羧甲基壳聚糖纳米粒子（CMCN，直径483 nm）与聚乙烯醇（PVA）共混溶液静电纺丝到改性细菌纤维素硫酸酯（BCS）膜表面，制备了直径约为100-500 nm的纳米纤维肝素样膜（CPBS）。SEM图像证实了在静电纺丝过程中CMCN被拉伸成纳米纤维。BCS在静电纺丝机捕收剂上的存在提高了CMCN/PVA溶液的可纺性。FTIR和XPS分析表明，CPBS膜表面存在单键SO 3-、COO-和单键OH基团，与肝素结构相似。CPBS膜保持亲水性，戊二醛交联的CPBS膜在水中稳定。凝血时间和血小板粘附实验反映了CPBS的抗凝作用。CPBS组APTT、TT和PT分别比血浆组提高116.0%、189.8%和50%（分别为67.4 s、16.2 s和48.4 s）。无血小板粘附于CPBS表面。根据接种在膜上的巨噬细胞的活化来确定炎症反应。
Heparin-like membranes (CPBS) with nanofibers (approximate diameters of 100–500 nm) were prepared through electrospinning of a blended solution of carboxymethyl chitosan nanoparticle (CMCN, diameters 483 nm) and poly (vinyl alcohol) (CMCN/PVA) onto the surface of modified bacterial cellulose sulfate (BCS) membranes. SEM images confirmed that the CMCN were stretched to nanofibers during electrospinning. The presence of BCS on the collector of electrospinning machine increased the spinnability of CMCN/PVA solution. FTIR and XPS measurement revealed that there were single bondSO3−, COO−, and single bondOH groups on the surface of CPBS membrane, expressing structural similarity to heparin. CPBS membranes maintained hydrophilicity and the glutaraldehyde crosslinked CPBS membrane was stable in water. The clotting time and platelet adhesion experiments expressed the anticoagulant properties of CPBS. The APTT, TT and PT of CPBS increased up to 116.0%, 189.8%, and 50% than those of the plasma, (67.4 s, 16.2 s, and 48.4 s, respectively). No platelets adhered onto the surface of CPBS. An inflammatory response was determined according to activation of the macrophages seeded onto the membranes.