Polymerase chain reaction for detection of Mycobacterium tuberculosis in epiretinal membrane in Eales' disease.
Polymerase chain reaction for detection of Mycobacterium tuberculosis in epiretinal membrane in Eales' disease.
复制标题
聚合酶链反应用于检测伊尔斯病视网膜前膜中的结核分枝杆菌。
DOI：
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发表时间：
2000
影响因子：
4.4
通讯作者：
J. Biswas
中科院分区：
文献类型：
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作者：
H. Madhavan;K. Therese;P. Gunisha;U. Jayanthi;J. Biswas
目的
Eales'病的病因可能是真菌。由于Eales病视网膜内表面形成视网膜前膜（ERM），因此它可能是最适合研究的眼内标本。因此，巢式聚合酶链反应（nPCR），检测结核分枝杆菌MPB64基因的ERM的档案标本的有充分记录的Eales'和非Eales'的患者，应用和结果进行比较。
方法
对nPCR技术进行标准化，并测定引物的敏感性和特异性。应用nPCR技术对23例Eales病患者和27例非感染性非Eales病患者的ERM组织进行福尔马林固定和石蜡包埋。
结果
nPCR技术对M.结核病基因组，灵敏度足以检测0.25 fg（对应于单个芽孢杆菌的存在）。23例Eales病患者中11例（47.8%）ERM阳性，27例对照者中3例（11.1%）阳性。结核病基因组两组之间的差异具有统计学意义（P = 0.001），表明该细菌与Eales病相关。
结论
证明了M.与对照相比，通过nPCR技术在显著数量的Eales'病的ERM中检测到结核DNA进一步强调了该细菌在这种神秘的临床病症的发病机制中的可能作用。
PURPOSE
Tuberculous etiology has been suggested in Eales' disease. Because epiretinal membrane (ERM) is formed on the inner surface of the retina in Eales' disease, it could be the most appropriate intraocular specimen for investigation. Therefore, a nested polymerase chain reaction (nPCR), which detects MPB64 gene of Mycobacterium tuberculosis on the archival specimens of ERM of well-documented Eales' and non-Eales' patients, was applied and the results compared.
METHODS
nPCR technique was standardized, and the sensitivity and specificity of the primers were determined. nPCR technique was applied to tissue sections obtained from formalin-fixed and paraffin-embedded tissues of ERM from 23 patients with Eales' disease and 27 noninfective and non-Eales' disease patients as controls.
RESULTS
nPCR technique was specific for M. tuberculosis genome and sensitive enough to detect 0.25 fg (corresponding to the presence of a single bacillus). Eleven (47.8%) ERM of 23 Eales' disease and 3 (11.1%) of 27 controls were positive for M. tuberculosis genome. The difference between the two groups was statistically significant (P = 0.001), indicating association of this bacterium with Eales' disease.
CONCLUSIONS
The demonstration of the presence of M. tuberculosis DNA by nPCR technique in significant number of ERM of Eales' disease compared with the controls further emphasizes the probable role of this bacterium in the pathogenesis of this enigmatic clinical condition.