Hardware triggering: maximizing speed and efficiency for live cell imaging

Hardware triggering: maximizing speed and efficiency for live cell imaging
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硬件触发:最大限度地提高活细胞成像的速度和效率

DOI:
10.1038/nmeth.f.403
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发表时间:
2017
期刊:
影响因子:
48
通讯作者:
Michael A. Davis
Michael A. Davis
中科院分区:
生物学1区
文献类型:
--
作者:
Michael A. Davis

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活细胞成像实验现在需要比以往更高的速度和更多的数据吞吐量。尼康仪器拥有强大的工具,可以通过硬件之间的直接信号对显微镜中的成像设备进行硬件触发。这可以最大限度地减少延迟、同步设备并减少样本的曝光。本应用说明解释了尼康的 NIS-Elements 硬件触发工作流程的运行方式,并详细介绍了其对常见延时采集例程的优势。
Live cell imaging experiments now require higher speeds and more data throughput than ever before. Nikon Instruments has robust tools that enable hardware triggering of imaging devices in microscopy via direct signaling between hardware. This minimizes delays, synchronizes devices, and reduces the exposure of specimens to light. This Application Note explains how Nikon's NIS-Elements hardware-triggering workflow operates, and details its benefits for common time-lapse acquisition routines.