Effects of Wuzi Yanzong pill and its diseassembled prescription on oxidative damage of skeletal muscle mitochondrial DNA in aged rats
Effects of Wuzi Yanzong pill and its diseassembled prescription on oxidative damage of skeletal muscle mitochondrial DNA in aged rats
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发表时间：
2001
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影响因子：
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通讯作者：
W. Xue
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作者：
W. Xue
？ 目的探讨五子养宗丸及其拆方对老年大鼠骨骼肌线粒体DNA（mtDNA）缺失、呼吸链复合物及ATP合成的影响。方法采用PCR、酶动力学和生物发光技术。结果24月龄大鼠骨骼肌mtDNA缺失量明显高于1月龄和6月龄大鼠，线粒体呼吸链复合物Ⅳ活性和ATP合成活性明显低于6月龄大鼠（P 0.05, 0.01）。乌子延总丸及其拆配方枸杞子、菟丝子能减少衰老大鼠线粒体DNA缺失，提高线粒体呼吸链复合物Ⅳ活性（P 0.05, 0.01），乌子延总丸还能提高衰老大鼠骨骼肌线粒体ATP合成（P 0.05）。结论乌子炎宗丸、枸杞子、菟丝子对衰老大鼠骨骼肌线粒体DNA氧化损伤有保护作用。
? Objective To investigate the effect of Wuzi Yangzong pill and its disassembled prescription on skeletal muscle mitochondrial DNA(mtDNA) deletion,respiratory chain complexes and ATP synthesis in aged rats.Methods By using PCR,enzyme kinetics and bioluminescence technique.Results The deletion of skeletal muscle mtDNA increased significantly in 24 month old rats compared with 1 and 6 month old rats,and the activities of mitochondrial respiratory chain complexes Ⅳ and ATP synthesis decreased significantly in 24 month old rats compared with 6 month old rats ( P 0 05,0 01).Wuzi Yanzong pill and its disassembled prescriptin of Fructus Lycii(wolfberry Fruit)and Semen Cusuctae(Dodder seed) could reduce the mitochondrial DNA deletions and raise the ativities of mitochondrial respiratory chain complexes Ⅳ in aged rats( P 0 05,0 01),Wuzi Yanzong pill could also raise the mitochondrial ATP synthesis in aged rat skeletal muscle( P 0 05).Conclusions Wuzi Yanzong pill,Fructus lycii and Semen Cusuctae have protective effect on oxidative damage of skeletal muscle mitochondrial DNA in aged rats.