A complex network of factors with overlapping affinities represses splicing through intronic elements.
A complex network of factors with overlapping affinities represses splicing through intronic elements.
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DOI：
10.1038/nsmb.2459
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发表时间：
2013-01
影响因子：
16.8
通讯作者：
Wang, Zefeng
中科院分区：
文献类型：
--
作者：
Wang, Yang;Xiao, Xinshu;Zhang, Jianming;Choudhury, Rajarshi;Robertson, Alex;Li, Kai;Ma, Meng;Burge, Christopher B.;Wang, Zefeng
为了更好地了解剪接调控，我们使用基于细胞的筛选来鉴定十种抑制内含子剪接的不同基序。每个基序都在另一种人类细胞类型和基因环境中得到验证，它们的存在与体内剪接变化相关。所有基序都表现出外显子剪接增强子或沉默子活性，并且根据这些基序的分布对这些基序进行分组，产生了具有不同背景依赖性活动模式的簇。鉴定了与每个基序相关的候选调节因子，恢复了 24 个已知和新型剪接调节因子。选定因素的特定领域足以赋予 ISS 活性。许多因子以相似的亲和力结合多个不同的基序，并且所有基序都被多个因子识别，揭示了蛋白质：RNA 相互作用的复杂、重叠网络。这种排列使得各个顺式元件能够根据存在的调节因子的范围在不同的细胞环境中发挥不同的作用。
To better understand splicing regulation, we used a cell-based screen to identify ten diverse motifs that inhibit splicing from intron. Each motif was validated in another human cell type and gene context, and their presence correlated with in vivo splicing changes. All motifs exhibited exonic splicing enhancer or silencer activity, and grouping these motifs based on their distributions yielded clusters with distinct patterns of context-dependent activity. Candidate regulatory factors associated with each motif were identified, recovering 24 known and novel splicing regulators. Specific domains in selected factors were sufficient to confer ISS activity. Many factors bound multiple distinct motifs with similar affinity, and all motifs were recognized by multiple factors, revealing a complex, overlapping network of protein:RNA interactions. This arrangement enables individual cis-element to function differently in distinct cellular contexts depending on the spectrum of regulatory factors present.
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DOI：
10.1111/j.1742-4658.2011.08077.x
发表时间：
2011-05
期刊：
The FEBS journal
影响因子：
--
作者：
Huang C;Xie MH;Liu W;Yang B;Yang F;Huang J;Huang J;Wu Q;Fu XD;Zhang Y
通讯作者：
Zhang Y
影响因子：
48
作者：
Katz, Yarden;Wang, Eric T.;Airoldi, Edoardo M.;Burge, Christopher B.
通讯作者：
Burge, Christopher B.
DOI：
10.1073/pnas.0700343104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Kashima, Tsuyoshi;Rao, Nishta;Manley, James L.
通讯作者：
Manley, James L.
影响因子：
10.5
作者：
KRAINER, AR;CONWAY, GC;KOZAK, D
通讯作者：
KOZAK, D
影响因子：
11.4
作者：
Hui, JY;Hung, LH;Bindereif, A
通讯作者：
Bindereif, A