6-Hydroxydaidzein Enhances Adipocyte Differentiation and Glucose Uptake in 3T3-L1 Cells
6-Hydroxydaidzein Enhances Adipocyte Differentiation and Glucose Uptake in 3T3-L1 Cells
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DOI：
10.1021/jf402694m
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发表时间：
2013-11-13
影响因子：
6.1
通讯作者：
Du, Yong-Li
中科院分区：
文献类型：
--
作者：
Chen, Li;Li, Qun-Yi;Du, Yong-Li
发酵大豆食品已被证明可以减少糖尿病的发病率，并改善胰岛素敏感性。6-羟基大豆苷元(6-HD)是从发酵大豆中分离得到的一种生物活性成分。在这项研究中，我们研究了6-HD对脂肪细胞分化和胰岛素刺激的葡萄糖摄取的影响及其相关机制。在我们的实验中，6-HD以剂量依赖的方式促进3T3-L1脂肪细胞分化和胰岛素刺激的葡萄糖摄取。此外，6-HD还增加了PPAR-γ基因的表达和PPAR-γ的转录活性。6-HD还可增加CCAAT/增强结合蛋白α(C/EBPα)的表达。6-HD对葡萄糖转运蛋白4(GLUT4)基因的表达没有影响，但促进了GLUT4蛋白向细胞膜的转运。我们的结果表明，6-HD通过增加C/EBPα的表达，并通过激活PPAR-γ促进GLUT4的移位，从而促进脂肪细胞的分化，改善胰岛素敏感性，提示6-HD在糖尿病治疗中具有潜在的应用前景。
Fermented soybean foods have been shown to reduce incidence of diabetes and improve insulin sensitivity. 6-Hydroxydaidzein (6-HD) is a bioactive ingredient isolated from fermented soybean. In this study, we examined the effects of 6-HD on adipocyte differentiation and insulin-stimulated glucose uptake, as well as the mechanisms involved. In our experiments, 6-HD enhanced 3T3-L1 adipocyte differentiation and insulin-stimulated glucose uptake in a dosage-dependent manner. In addition, 6-HD increased peroxisome proliferator-activated receptor gamma (PPAR gamma) gene expression and PPAR gamma transcriptional activity. 6-HD increased CCAAT/enhanced binding protein alpha (C/EBP alpha) expression as well. Although having no effects on glucose transporter type 4 (GLUT4) gene expression, 6-HD facilitated GLUT4 protein translocation to the cell membranes. Our results indicate that 6-HD exhibited the actions of promoting adipocyte differentiation and improving insulin sensitivity by increasing the expression of C/EBP alpha and facilitating the translocation of GLUT4 via the activation of PPAR gamma, suggesting that 6-HD can be promising in diabetes management.