Production of the Fos protein after contextual fear conditioning of C57BL/6N mice
Production of the Fos protein after contextual fear conditioning of C57BL/6N mice
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DOI：
10.1016/s0006-8993(97)01266-3
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发表时间：
1998-02-16
期刊：
影响因子：
2.9
通讯作者：
Spiess, J
中科院分区：
文献类型：
--
作者：
Milanovic, S;Radulovic, J;Spiess, J
选择雄性 C57BL/6N 小鼠来测定获得背景依赖性恐惧期间以及重新暴露于条件环境后的 Fos 产生。恐惧调节是通过将小鼠单次暴露在环境中然后进行电击来诱导的。对照组由仅暴露于环境（环境组）或立即电击的小鼠组成。当条件反射后 24 小时测量情境保留（保留测试 1）时，观察到显着的情境泛化。然而，当动物在调节后第 2-5 天暴露于不同的环境，然后在第 6 天测试保留（保留测试 2）时，泛化能力显着降低。训练后，处于恐惧条件下的小鼠的海马体、内侧杏仁核和顶叶体感皮层产生的 Fos 水平高于立即受到电击的小鼠。两个休克组在杏仁核中央核中产生的 Fos 明显多于背景组。在保留测试 1 后，与两个对照组相比，恐惧条件小鼠在海马体和中央杏仁核中产生了更多的 Fos。然而，在保留测试2后，所有组都表现出类似的低Fos产量。结果表明，海马、杏仁核中央和内侧核以及体感顶叶皮层同时产生的Fos与小鼠获得条件性恐惧的能力密切相关。相比之下，保留测试后 Fos 的产生与条件性恐惧的表达无关。 (C) 1998 Elsevier Science B.V.
Male C57BL/6N mice were chosen to determine Fos production during acquisition of context-dependent fear and after re-exposure to the conditioning context. Fear-conditioning was induced by a single exposure of mice to a context followed by an electric shock. Control groups consisted of mice exposed to context only (Context group) or to an immediate electric shock. When contextual retention was measured 24 h after conditioning (retention test 1), significant contextual generalization was observed. However, when animals were exposed to a different context from days 2-5 after conditioning and then tested for retention on day 6 (retention test 2), generalization was markedly reduced. After the training, the fear-conditioned mice produced higher Fos levels than mice exposed to an immediate shock in the hippocampus, medial amygdaloid nucleus and parietal somatosensory cortex. Both shock groups produced significantly more Fos than the Context group in the central nucleus of the amygdala. After retention test 1, fear-conditioned mice generated more Fos in the hippocampus and central amygdaloid nucleus than the two control groups. However, all groups exhibited similarly low Fos production after retention test 2. The results demonstrated that simultaneous Fos production in the hippocampus, central and medial nuclei of amygdala and somatosensory parietal cortex closely paralleled the ability of mice to acquire conditioned fear. In contrast, Fos production after the retention tests did not correlate with the expression of conditioned fear. (C) 1998 Elsevier Science B.V.