A dual‐specific Glu‐tRNAGln and Asp‐tRNAAsn amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans
A dual‐specific Glu‐tRNAGln and Asp‐tRNAAsn amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans
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双特异性 Glu-tRNAGln 和 Asp-tRNAAsn 酰胺转移酶参与解码氧化亚铁硫杆菌中的谷氨酰胺和天冬酰胺密码子
DOI：
10.1016/s0014-5793(01)02600-x
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发表时间：
2001
期刊：
影响因子：
3.5
通讯作者：
O. Orellana
中科院分区：
文献类型：
--
作者：
J. Salazar;R. Zúñiga;Gregory Raczniak;H. Becker;D. Söll;O. Orellana
本研究克隆了氧化亚铁硫杆菌谷氨酰转氨酶三个亚基的gatC、gatA和gatB基因，并在大肠杆菌中进行了表达。在枯草芽孢杆菌中，这三个巨细胞基因是在一个类似操纵子的结构中组织起来的。氧化亚铁菌。异源过表达的酶将Glu-tRNAGln转化为Gln-tRNAGln，Asp-tRNAAsn转化为Asn-tRNAAsn。生化分析表明，谷氨酰胺-tRNA合成酶和天冬酰胺-tRNA合成酶均不存在。但谷氨酰-tRNA合成酶和天冬氨酰-tRNA合成酶存在于生物体中。这些数据表明，转酰胺化途径负责形成Gln-tRNA和Asn-tRNA Ina。氧化亚铁菌。
ThegatC,gatAandgatBgenes encoding the three subunits of glutamyl‐tRNAGlnamidotransferase fromAcidithiobacillus ferrooxidans, an acidophilic bacterium used in bioleaching of minerals, have been cloned and expressed inEscherichia coli. As inBacillus subtilisthe threegatgenes are organized in an operon‐like structure inA. ferrooxidans. The heterologously overexpressed enzyme converts Glu‐tRNAGlnto Gln‐tRNAGlnand Asp‐tRNAAsnto Asn‐tRNAAsn. Biochemical analysis revealed that neither glutaminyl‐tRNA synthetase nor asparaginyl‐tRNA synthetase is present inA. ferrooxidans, but that glutamyl‐tRNA synthetase and aspartyl‐tRNA synthetase enzymes are present in the organism. These data suggest that the transamidation pathway is responsible for the formation of Gln‐tRNA and Asn‐tRNA inA. ferrooxidans.