IDENTIFICATION OF RFLP MARKERS CLOSELY LINKED TO THE BOLTING GENE-B AND THEIR SIGNIFICANCE FOR THE STUDY OF THE ANNUAL HABIT IN BEETS (BETA-VULGARIS L)
IDENTIFICATION OF RFLP MARKERS CLOSELY LINKED TO THE BOLTING GENE-B AND THEIR SIGNIFICANCE FOR THE STUDY OF THE ANNUAL HABIT IN BEETS (BETA-VULGARIS L)
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DOI：
10.1007/bf01253996
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发表时间：
1994-08-01
影响因子：
5.4
通讯作者：
JUNG, C
中科院分区：
文献类型：
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作者：
BOUDRY, P;WIEBER, R;JUNG, C
甜菜的年度习性是由于完全或部分缺乏春化要求，并可能导致甜菜作物的严重问题。甜菜的绝对春化需求由连锁群I中的一个主效基因B(抽苔)控制，该基因与红色下胚轴颜色基因R连锁。BB型一年生食性的外显性受环境因素和遗传因素的共同影响。利用限制性片段长度多态(RFLP)分析，对一年生性部分外显的回交后代进行了主效基因B在I连锁群的精确定位。B和R之间的连锁值与先前的估计值一致。使用最接近的RFLP标记(pKP591：3.8重组单位)，我们可以估计该回交一年生习性的外显率为0.62。在作为回交母本的沿海野生甜菜中发现了假亲和性的证据：自交率估计为7%。
The annual habit in beet is due to complete or partial absence of the vernalization requirement and can cause severe problems in the beet crop. The absolute vernalization requirement in beet is controlled by a major gene B (bolting), known to be linked to the gene R (red hypocotyl color), in linkage group I. Segregation for the B and R genes was studied in several beet progenies. Penetrance of the annual habit in Bb genotypes was affected by both environmental and genetic factors. The precise location in linkage group I of the major gene B was found by restriction fragment length polymorphism (RFLP) analysis in a back-cross progeny exhibiting partial penetrance of the annual habit. The linkage value between B and R was in good accordance with previous estimations. Use of the closest RFLP marker (pKP591: 3.8 recombination units) allowed us to estimate the penetrance of the annual habit in this back-cross as 0.62. Evidence of pseudo-compatibility was found in the wild coastal beet (Beta vulgaris ssp maritima) used as the mother plant of the back-cross: the selfing rate was estimated as 7%.