DETAILED PHYSICAL MAP AND SET OF OVERLAPPING CLONES COVERING THE GENOME OF THE ARCHAEBACTERIUM HALOFERAX-VOLCANII DS2
DETAILED PHYSICAL MAP AND SET OF OVERLAPPING CLONES COVERING THE GENOME OF THE ARCHAEBACTERIUM HALOFERAX-VOLCANII DS2
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DOI：
10.1016/0022-2836(91)90493-p
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发表时间：
1991-12-05
影响因子：
5.6
通讯作者：
DOOLITTLE, WF
中科院分区：
文献类型：
--
作者：
CHARLEBOIS, RL;SCHALKWYK, LC;DOOLITTLE, WF
“自下而上”和“自上而下”作图的综合方法产生了覆盖 4140 千碱基对 (kbp)Haloferax volcaniiDS2 基因组的 96% 的最小重叠粘粒克隆集和完全封闭的物理图谱。该基因组分为五个复制子：一个 2920 kbp 的染色体和四个质粒，分别为 690 kbp (pHV4)、442 kbp (pHV3)、86 kbp(pHV1) 和 6·4 kbp (pHV2)。构建了 6 个不常切割的限制性内切酶的限制性图谱，代表克隆 DNA 中总共 903 个位点。我们在图谱上放置了两个核糖体 RNA 操纵子、7 S RNA 和 RNaseP RNA 的基因以及 22 个蛋白质编码基因。限制性位点频率显示基因组不同部分的显着差异。高位点密度区域对应于盐细菌卫星或 FII DNA，其中包括染色体的两个小区域，即质粒 pHV1 和 pHV2，以及一半的 pHV4，但不包括 pHV3。
An integrated approach of “bottom up” and “top down” mapping has produced a minimal set of overlapping cosmid clones covering 96% of the 4140 kilobase-pairs (kbp)Haloferax volcaniiDS2 genome and a completely closed physical map. This genome is partitioned into five replicons: a 2920 kbp chromosome and four plasmids, of 690 kbp (pHV4), 442 kbp (pHV3), 86 kbp(pHV1) and 6·4 kbp (pHV2). A restriction map for six infrequently-cutting restriction enzymes was constructed, representing a total of 903 sites in the cloned DNA. We have placed the two ribosomal RNA operons, the genes for 7 S RNA and for RNaseP RNA and 22 protein-coding genes on the map. Restriction site frequencies show significant variation in different portions of the genome. The regions of high site density correspond to halobacterial satellite or FII DNA which includes two small regions of the chromosome, the plasmids pHV1 and pHV2, and half of pHV4, but not pHV3.