Comparison of the effects of an organic and an inorganic form of selenium on a mammary carcinoma cell line.
Comparison of the effects of an organic and an inorganic form of selenium on a mammary carcinoma cell line.
复制标题
DOI：
10.1093/carcin/15.2.183
复制
发表时间：
1994-02
期刊：
影响因子：
4.7
通讯作者：
H. Thompson;A. Wilson;Junxuan Lu;Meenakshi Singh;Cheng Jiang;P. Upadhyaya;K. El-Bayoumy;C. Ip
中科院分区：
文献类型：
--
作者：
H. Thompson;A. Wilson;Junxuan Lu;Meenakshi Singh;Cheng Jiang;P. Upadhyaya;K. El-Bayoumy;C. Ip
开发具有更强抑癌活性且耐受性良好的新化合物仍然是涉及硒的化学预防研究的重点。其中一个化合物，1，-4-苯-双（亚甲基）硒氰酸酯（p-XSC），是具有这些特征的一系列有机硒化合物的代表。在本研究中，我们比较了p-XSC对小鼠乳腺癌细胞系的影响，并将其与亚硒酸钠进行了比较，亚硒酸钠已被证明具有生长抑制作用。p-XSC处理导致细胞内硒的积累比等量亚硒酸盐处理多3- 6倍，细胞能够更好地耐受来自p-XSC的更高细胞水平的硒。暴露24小时后，两种化合物均导致细胞数量呈剂量依赖性减少。亚硒酸盐和p-XSC也引起细胞凋亡死亡的剂量依赖性增加。这种效果在治疗后5小时内观察到。p-XSC对细胞凋亡的影响比亚硒酸盐更明显，特别是在20微米水平下。硒化合物对细胞凋亡的诱导可能是其化学预防作用的部分原因。
The development of new compounds with greater cancer inhibitory activity and that are well tolerated continues to be a priority in chemoprevention research involving selenium. One compound, 1,-4-phenylene-bis(methylene)selenocyanate (p-XSC), is representative of a series of organoselenium compounds with these characteristics. In this study, the effects of p-XSC on a mouse mammary carcinoma cell line were compared to those of sodium selenite, which has been shown to be growth inhibitory. Treatment with p-XSC caused a 3- to 6-fold greater accumulation of selenium within cells than did treatment with equivalent amounts of selenite and cells were able to better tolerate higher cellular levels of selenium derived from p-XSC. Both compounds resulted in a dose-dependent reduction in cell number after 24 h of exposure. Selenite and p-XSC also caused a dose-dependent increase in cell death by apoptosis. This effect was observed within 5 h of treatment. The effect of p-XSC on apoptosis was more pronounced than that of selenite, especially at the 20 microM level of exposure. The induction of apoptosis by selenium compounds may partially account for their chemopreventive activity.