On the number of protein-protein interactions in the yeast proteome
On the number of protein-protein interactions in the yeast proteome
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DOI：
10.1093/nar/gkg466
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发表时间：
2003-07-15
影响因子：
14.9
通讯作者：
Grigoriev, A
中科院分区：
文献类型：
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作者：
Grigoriev, A
使用两种不同的方法，我们估计，平均有大约五个相互作用的合作伙伴，每个蛋白质的酵母酿酒酵母的蛋白质组。在第一种方法中，我们使用了一种新的方法，通过伯努利过程来模拟采样重叠，比较了两个独立的酵母双杂交相互作用筛选的结果，并测试了估计的鲁棒性。当从分析中去除蛋白质相互作用网络中的三个最高度连接的节点时，获得了每个蛋白质五个相互作用物的最稳定估计值（如果保留这些节点，则每个蛋白质八个相互作用物）。在第二种方法中，我们分析了一个已发表的高置信度子集的假定相互作用的数据从多个来源，包括大规模的双杂交筛选，复杂的纯化，合成致死，相关的基因表达，计算预测和以前的注释。引人注目的是，估计每个蛋白质又有五个相互作用物。这些估计表明在酵母中有16000 - 26000个不同的相互作用对，不包括同型相互作用。我们还讨论了估计同型相互作用率的方法。
Using two different approaches, we estimated that on average there are about five interacting partners per protein in the proteome of the yeast Saccharomyces cerevisiae. In the first approach, we used a novel method to model sampling overlap by a Bernoulli process, compared the results of two independent yeast two-hybrid interaction screens and tested the robustness of the estimate. The most stable estimate of five interactors per protein was obtained when the three most highly connected nodes in the protein interaction network were removed from the analysis (eight interactors per protein if those nodes were kept). In the second approach, we analysed a published high-confidence subset of putative interaction data obtained from multiple sources, including large-scale two-hybrid screens, complex purifications, synthetic lethals, correlated gene expression, computational predictions and previous annotations. Strikingly, the estimate was again five interactors per protein. These estimates suggest a range of similar to16 000-26 000 different interaction pairs in the yeast, excluding homotypic interactions. We also discuss the approaches to estimating the rate of homotypic interactions.