Diversity and evolution of coral fluorescent proteins.
Diversity and evolution of coral fluorescent proteins.
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DOI：
10.1371/journal.pone.0002680
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发表时间：
2008-07-16
期刊：
影响因子：
3.7
通讯作者：
Matz MV
中科院分区：
文献类型：
--
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
类绿色荧光蛋白（FPs）是造礁珊瑚（珊瑚纲，石珊瑚目）颜色的关键决定因素，作为潜在的基因编码荧光标记物备受关注。在此我们报道了来自珊瑚的另外40种绿色荧光蛋白家族成员。珊瑚荧光蛋白有三个主要的旁系同源谱系。其中一个存在于所有取样的珊瑚科中，负责产生非荧光的紫蓝色，而另外两个谱系各自进化出了一整套典型的珊瑚荧光色（青色、绿色和红色），并且在珊瑚类群之间发生了分化。在新克隆的蛋白中，有来自福氏刺孔珊瑚（蜂巢珊瑚科）的一种“红色色素”类型和来自尖枝列孔珊瑚（鹿角珊瑚科）的粉色色素蛋白，它们都独立于其他珊瑚色素蛋白进化而来。有几种青色荧光蛋白具有一种新型的激发光谱，表明其发色团处于中性基态，这是由残基E167导致的（根据维多利亚多管发光水母的绿色荧光蛋白编号）。来自轴孔珊瑚的色素蛋白是一种不寻常的蓝色而非紫色，这是由于两个突变：S64C和S183T。我们应用一种新的概率抽样方法来重建所有珊瑚荧光蛋白的共同祖先，以及蜂巢珊瑚亚目三种主要荧光色更衍生的共同祖先。这两种蛋白都是绿色的，就像在珊瑚纲之外的其他地方发现的那样。有趣的是，所有珊瑚的祖先蛋白中有相当一部分的发色团显然锁定在非荧光的中性状态，这可能反映了在珊瑚荧光蛋白历史早期使颜色快速多样化的过渡阶段。我们的结果强调了珊瑚颜色多样性趋同或平行进化的程度，为导致颜色多样化的进化过程的实验研究提供了基础，并能够对不同颜色的结构决定因素进行比较分析。
GFP-like fluorescent proteins (FPs) are the key color determinants in reef-building corals (class Anthozoa, order Scleractinia) and are of considerable interest as potential genetically encoded fluorescent labels. Here we report 40 additional members of the GFP family from corals. There are three major paralogous lineages of coral FPs. One of them is retained in all sampled coral families and is responsible for the non-fluorescent purple-blue color, while each of the other two evolved a full complement of typical coral fluorescent colors (cyan, green, and red) and underwent sorting between coral groups. Among the newly cloned proteins are a “chromo-red” color type from Echinopora forskaliana (family Faviidae) and pink chromoprotein from Stylophora pistillata (Pocilloporidae), both evolving independently from the rest of coral chromoproteins. There are several cyan FPs that possess a novel kind of excitation spectrum indicating a neutral chromophore ground state, for which the residue E167 is responsible (numeration according to GFP from A. victoria). The chromoprotein from Acropora millepora is an unusual blue instead of purple, which is due to two mutations: S64C and S183T. We applied a novel probabilistic sampling approach to recreate the common ancestor of all coral FPs as well as the more derived common ancestor of three main fluorescent colors of the Faviina suborder. Both proteins were green such as found elsewhere outside class Anthozoa. Interestingly, a substantial fraction of the all-coral ancestral protein had a chromohore apparently locked in a non-fluorescent neutral state, which may reflect the transitional stage that enabled rapid color diversification early in the history of coral FPs. Our results highlight the extent of convergent or parallel evolution of the color diversity in corals, provide the foundation for experimental studies of evolutionary processes that led to color diversification, and enable a comparative analysis of structural determinants of different colors.
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影响因子：
3.4
作者：
Agmon, N
通讯作者：
Agmon, N
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
DOI：
10.1073/pnas.97.22.11984
发表时间：
2000-10-24
影响因子：
11.1
作者：
Baird, GS;Zacharias, DA;Tsien, RY
通讯作者：
Tsien, RY
影响因子：
56.9
作者：
Lesser, MP;Mazel, CH;Falkowski, PG
通讯作者：
Falkowski, PG
影响因子：
3.3
作者：
Agmon, Noam
通讯作者：
Agmon, Noam