Defocus-gradient corrected back-projection
Defocus-gradient corrected back-projection
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DOI：
10.1016/s0304-3991(00)00005-x
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发表时间：
2000-07-01
期刊：
影响因子：
2.2
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Jensen, GJ;Kornberg, RD
从冷冻电子显微镜图像的二十面体病毒的三维重建已经达到分辨率，显微镜的景深是一个重要的分辨率限制因素。这里提出的分析处理表明，保持限制的深度如何可以理解为一个包络函数，它在实际达到数值景深之前就开始逐渐衰减信号。本文描述了对众所周知的反投影重建算法的一种简单修改，称为离焦梯度校正反投影，它沿离焦梯度计算校正对比度传递函数。计算机仿真验证了该算法如何有效地消除了持有深度的限制。(C) 2000 Elsevier Science B.V.版权所有
Three-dimensional reconstructions of icosahedral viruses from cryoelectron microscope images have reached resolutions where the microscope depth of field is a significant resolution-limiting factor. An analytical treatment presented here shows how the depth of held limitation can be understood as an envelope function which gradually attenuates the signal, starting well before the numerical depth of field is actually reached. A simple modification to the well-known back-projection reconstruction algorithm is described, called the defocus-gradient corrected back-projection, which computationally corrects for the contrast transfer function along a defocus gradient. Computer simulations demonstrate how the algorithm effectively eliminates the depth of held limitation. (C) 2000 Elsevier Science B.V. All rights reserved.