Co-occurrence of aflatoxin B1, B2, G1, G2 and ochrotoxin A in Glycyrrhiza uralensis analyzed by HPLC-MS/MS
Co-occurrence of aflatoxin B1, B2, G1, G2 and ochrotoxin A in Glycyrrhiza uralensis analyzed by HPLC-MS/MS
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HPLC-MS/MS 分析乌拉尔甘草中黄曲霉毒素 B1、B2、G1、G2 和赭曲霉毒素 A 的共存
DOI：
10.1016/j.foodcont.2012.11.028
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发表时间：
2013-07-01
期刊：
影响因子：
6
通讯作者：
Ma, Xiujing
中科院分区：
文献类型：
--
作者：
Wei, Riwei;Qiu, Feng;Ma, Xiujing
甘草在食品和制药工业中广泛用作添加剂，乌拉尔甘草（Glycyrrhiza uralensis）是中国甘草的主要来源之一。乌拉尔甘草在生长、采集、运输，尤其是储存过程中可能受到霉菌毒素污染。首次开发了一种快速且高灵敏度的方法，采用高效液相色谱 - 串联质谱法（HPLC - MS/MS）测定乌拉尔甘草中的黄曲霉毒素（AFs）和赭曲霉毒素A（OTA）。由于所研究的基质复杂且颜色较深，该方法基于使用甲醇/水混合液（80:20，v/v）超声提取，然后用免疫亲和柱（IAC）净化。黄曲霉毒素B - 1、B - 2、G₁、G₂和OTA的定量限（LOQs）在信噪比为10:1的情况下分别为0.020、0.015、0.010、0.015和0.010μg/kg。所有目标分析物均呈现良好的线性，在各自的线性范围内相关系数均高于0.995。在中国不同地区采集的总共15份乌拉尔甘草样品中，6份因储存不当发霉，9份良好，无任何可见的霉菌污染。结果表明，几乎所有样品都受到AFs和OTA污染。发霉样品中AFs和OTA的含量高于无明显发霉的样品，其中两份发霉样品超过了欧盟法规对甘草中OTA设定的最大限量。这是中国首次关于乌拉尔甘草中AFs和OTA共同存在的报道。版权所有（C）2012，爱思唯尔有限公司出版。保留所有权利。
Licorice is widely used as additives in food and pharmaceutical industries and Glycyrrhiza uralensis is one of the main sources of licorice in China. G. uralensis may be contaminated by mycotoxins during growth, collection, transportation and, especially, storage. A rapid and highly sensitive method was firstly developed to determine aflatoxins (AFs) and ochratoxin A (OTA) in G. uralensis using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Due to the complexity and high color of the investigated matrices, the proposed method is based on sonication extraction using a methanol/water mixture (80:20, v/v) followed by immunoaffinity column (IAC) cleanup. The limits of quantification (LOQs) of aflatoxins B-1, B-2, G(1), G(2) and OTA were 0.020, 0.015, 0.010, 0.015 and 0.010 mu g/kg based on a signal-to-noise ratio of 10: 1, respectively. Good linearity was observed for all the analytes of interest, with correlation coefficients all higher than 0.995 within their respective linear ranges. Among the total of 15 G. uralensis samples collected from different areas in China, 6 were moldy due to inappropriate storage and 9 were fine without any visible mold contamination. The results showed that almost all the samples were contaminated with AFs and OTA. The AFs and OTA levels found in the moldy samples were higher than those not visibly moldy and two of the moldy samples exceeded the maximum limit set for OTA in licorice by EU regulation. This is the first report of the co-occurrence of AFs and OTA in G. uralensis in China. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.