ON THE ACTIVITIES OF ESCHERICHIA-COLI EXONUCLEASE-III
ON THE ACTIVITIES OF ESCHERICHIA-COLI EXONUCLEASE-III
复制标题
DOI：
10.1006/abio.1993.1114
复制
发表时间：
1993-03-01
影响因子：
2.9
通讯作者：
HOHEISEL, JD
中科院分区：
文献类型：
--
作者：
HOHEISEL, JD
大肠杆菌的核酸外切酶III （Exo III）是分子生物学中常用的一种dna修饰酶。这里描述的实验是为了可靠地控制外切酶活性和了解更多关于酶的特异性而进行的。考察了温度（包括热失活）、Exo III和NaCl浓度、3′末端的浓度和形状等因素对Exo III活性的影响。双链DNA被发现是酶活性的竞争性抑制剂。有4个核苷酸3 '突起对Exo III消化敏感。此外，还研究了基因删除的同步性。讨论了所提出的活性机制的含义。
Exonuclease III (Exo III) of Escherichia coli is a DNA-modifying enzyme very frequently used in molecular biology. The experiments described here were carried out with the aims of reliably controlling exonuclease activity and of learning more about the enzyme′s specificities. The dependence of Exo III activity on factors such as temperature (including heat inactivation), the concentrations of Exo III and NaCl, and the concentration and shape of 3′ termini was investigated. Double stranded DNA was found to be a competitive inhibitor of the enzyme activity. Some four nucleotide 3′ protrusions were shown to be sensitive to Exo III digestion. The synchronism of deletion was also examined. Implications for the proposed mechanism of activity are discussed.