Heat stress in pigs is accompanied by adipose tissue-specific responses that favor increased triglyceride storage
Heat stress in pigs is accompanied by adipose tissue-specific responses that favor increased triglyceride storage
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DOI：
10.2527/jas.2015-0084
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发表时间：
2016-05-01
影响因子：
3.3
通讯作者：
Ajuwon, K. M.
中科院分区：
文献类型：
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作者：
Qu, H.;Yan, H.;Ajuwon, K. M.
热应激(HS)对猪各方面的生产性能都有负面影响。虽然已知肝脏、骨骼肌和肠道的某些组织特异性反应，但关于脂肪组织内的反应的信息很少。因此，本研究的目的是描述猪在HS过程中的脂肪组织反应。将30头杂交(Ossabaw×Duroc×Landrace)猪随机分成3个处理，每组7 d，分别为：1)对照+Libitum饲喂(CON)，室温为20℃+/-1℃；2)成对饲喂(PF)，室温为CON处理，但配对饲喂HS猪；与普通猪相比，HS猪采食量减少，皮肤温度和呼吸频率升高(P<；0.01)。HS猪的血尿素氮水平显著高于普通猪(P=0.01)。在皮下和肠系膜脂肪组织中，HS组大鼠皮下脂肪组织和肠系膜脂肪组织中PCK1的表达水平均高于对照组和PF组(P<；0.01)。热应激还导致肠系膜脂肪中热休克蛋白70(HSP70；P=0.067)和CCAT/增强子结合同源蛋白(CHOP)含量增加。综上所述，HS可诱导脂肪组织中PCK1的表达，提示HS下脂肪储存量增加可能与甘油合成增加有关。
Heat stress (HS) negatively affects all aspects of performance in pigs. Although certain tissue-specific responses in the liver, skeletal muscle, and intestine are known, there is paucity of information on responses within the adipose tissue. Therefore, the objective of this study was to delineate adipose tissue responses during HS in pigs. Thirty crossbred (Ossabaw x Duroc x Landrace) pigs were assigned to 3 treatments for 7 d. Treatments were 1) control and libitum fed (CON) with room temperature set at 20 degrees C +/- 1 degrees C, 2) pair fed (PF) with room temperature as the CON treatment but pair fed to HS pigs, and 3) HS with room temperature 35 degrees C +/- 1 degrees C and ad libitum access to feed. Compared with CON pigs, HS pigs had decreased feed intake and elevated skin temperature and respiration rate (P < 0.01). Blood urea nitrogen was higher (P = 0.01) in HS pigs compared with CON pigs only in males. In both subcutaneous and mesenteric adipose tissue, mRNA abundance of phosphoenolpyruvate carboxykinase (PCK1) was more elevated (P < 0.01) in HS groups compared with the CON and PF groups. Heat stress also caused increased heat shock protein 70 (HSP70; P = 0.067) and CCAT/enhancer-binding homologous protein (CHOP) content (P < 0.05) in the mesenteric fat compared with the CON treatment. In conclusion, induction of PCK1 expression in adipose tissue by HS suggests elevated glyceroneogenesis might be involved in the increased fat storage in pigs under HS.