Determining the limits and confounders for the 2-pentyl furan breath test by gas chromatography/mass spectrometry
Determining the limits and confounders for the 2-pentyl furan breath test by gas chromatography/mass spectrometry
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DOI：
10.1016/j.jchromb.2011.08.010
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发表时间：
2011-09-15
影响因子：
3
通讯作者：
Scott-Thomas, Amy
中科院分区：
文献类型：
--
作者：
Bhandari, Shrawan;Chambers, Stephen;Scott-Thomas, Amy
烟曲霉产生2-戊基呋喃（2-PF），这是一种挥发性化合物，许多其他病原体或正常人体代谢都不会产生。2-戊基呋喃已在侵袭性曲霉病（IA）患者的呼吸中通过SPME预浓缩结合CG/MS检测到，提供了一种有吸引力的诊断试验的可能性。对峰积分的检出限（LOD）和定量（LOQ）分别进行了统计和经验评估。在45种受检食物中，有10种检出2-戊基呋喃。豆奶（3个品牌中的3个）的含量最高，南瓜、花生、燕麦片、sure Plus (R)罐装芦笋、罐装豆类和一种蔬菜（Marmiten）的含量较低。在用于治疗IA的抗真菌药物或常用化妆品中检测不到2-PF。从饮食中摄取不含富含2-PF食物的健康受试者中提取的空腹和非空腹样本中，上午和下午的2-PF呼吸水平没有差异。2-戊基呋喃水平在用豆浆漱口后立即出现在呼吸样本中（P < 0.001），在一些受试者摄入豆浆并用水漱口后也出现在呼吸样本中。2-PF呼气测试可以在不禁食的情况下进行，也可以在特定的时间进行，前提是从摄入含有2-PF的产品开始，漱口30分钟或更长时间。(C) 2011 Elsevier B.V.版权所有
Aspergillus fumigatus produces 2-pentyl furan (2-PF), a volatile compound not produced by many other pathogens or normal human metabolism. 2-Pentyl furan has been detected in the breath of patients with invasive aspergillosis (IA) by SPME pre-concentration coupled with CG/MS providing the possibility of an attractive diagnostic test. The limit of detection (LOD) and quantification (LOQ) for peak integration were assessed both statistically and empirically respectively. 2-Pentyl furan was detected from 10 of 45 food stuffs tested. Levels were highest from soymilk (3 of 3 brands), lower from pumpkin, peanuts, rolled oats 2, Ensure Plus (R) tinned asparagus, tinned beans and a vegetable exact (Marmiten (TM)). No 2-PF was detectable in anti-fungal medications used to treat IA or commonly used cosmetics tested. There was no difference in 2-PF breath levels between morning and afternoon or fasting and non fasting samples taken from healthy subjects eating a diet without 2-PF rich foods. 2-Pentyl furan levels were present in breath samples immediately after a mouth rinse with soy milk (P < 0.001), and in some subjects after ingesting soy milk and rinsing their mouth with water. The breath test for 2-PF can be conducted without an overnight fast or at a specified time provided the mouth has been rinsed 30 min or more from when 2-PF containing products have been ingested. (C) 2011 Elsevier B.V. All rights reserved.