Brain Membrane Proteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers
Brain Membrane Proteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers
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脑膜蛋白质组和磷酸化蛋白质组揭示了与蜜蜂工蜂的护理和觅食行为相关的分子基础
DOI：
10.1021/acs.jproteome.7b00371
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发表时间：
2017-10-01
影响因子：
4.4
通讯作者：
Li, Jianke
中科院分区：
文献类型：
--
作者：
Han, Bin;Fang, Yu;Li, Jianke
蜜蜂的大脑是调节蜜蜂复杂社会行为的重要器官，包括学习和记忆。关于脑膜蛋白及其磷酸化是年龄相关的行为多态化的基础的知识仍然缺乏。意大利蜜蜂（Italian bee，ITB）和皇家果冻蜂（Royal Jelly bee，RJB）是从ITB中筛选出的一个40年来产量增加的品系。共鉴定出1079个膜蛋白组，417个独特的磷酸化位点位于179个膜蛋白组中，主要由MAPK、CDK和CK 2的激酶家族磷酸化。脑细胞膜蛋白质组和磷酸化蛋白质组的高分辨动力学表明了它们与蜜蜂工蜂成年期神经生物学需求的相关性。为了刺激新出现的蜜蜂（NEB）中未成熟的脑细胞发育，与碳水化合物，核苷和脂质代谢相关的功能类通过上调蛋白质表明它们在驱动脑细胞成熟中的作用增强。与幼蜂相比，在哺育蜂和觅食蜂中，膜蛋白和磷蛋白的表达量均有所增加，这些蛋白表达量的增加丰富了与信号转导相关的通路，表明它们在维持哺育和觅食活动中密集的信息处理过程中具有重要意义。值得注意的是，RJB已经形成了独特的膜蛋白质组和磷酸化蛋白质组设置，以巩固护理和觅食行为，以应对数十年的选择，从而提高RJ产量。在RJB NBs中，丰富的磷脂酰肌醇信号转导和花生四烯酸代谢途径表明，幼虫信息素刺激的嗅觉感觉更强。在RJB FBs中，与信号处理相关的丰富途径，如囊泡运输中的SNARE相互作用，writ信号传导，TGF-β信号传导以及牛磺酸和亚牛磺酸代谢，表明神经敏感性增强，从而引发更强的花粉收集倾向。我们的数据获得了一个新的见解膜蛋白质组和磷酸化蛋白质组驱动的蜜蜂行为的大脑调节，这是潜在的有用的蜜蜂和其他社会昆虫的神经生物学研究。
The brain is a vital organ in regulating complex social behaviors of honeybees including learning and memory. Knowledge of how brain membrane proteins and their phosphorylation underlie the age-related behavioral polyethism is still lacking. A hitherto age resolved brain membrane proteome and phosphoproteome were reported in adult worker bees from two strains of honeybee (Apis mellifera ligustica): Italian bee (ITB) and Royal Jelly bee (RJB), a line selected from ITB for increased RJ outputs over four decades. There were 1079 membrane protein groups identified, and 417 unique phosphosites were located in 179 membrane protein groups mainly phosphorylated by kinase families of MAPKs, CDKs, and CK2. Age-resolved dynamics of brain membrane proteome and phosphoproteome are indicative of their correlation with the neurobiological requirements during the adult life of honeybee workers. To stimulate immature brain cell development in newly emerged bees (NEBs), the enriched functional classes associated with metabolism of carbohydrates, nucleosides, and lipids by the up-regulated proteins suggest their enhanced role in driving cell maturity of the brain. In nurse bees (NBs) and forager bees (FBs), a higher number of membrane proteins and phosphoproteins were expressed as compared with in the young stages, and the enriched signal-transduction-related pathways by the up-regulated proteins suggest their significances in sustaining the intensive information processing during nursing and foraging activities. Notably, RJB has shaped unique membrane proteome and phosphoproteome settings to consolidate nursing and foraging behaviors in response to decades of selection underpinning the elevated RJ yields. In RJB NBs, the enriched pathways of phosphatidylinositol signaling and arachidonic acid metabolism indicate a stronger olfaction sensation in response to larval pheromone stimulation. In RJB FBs, the enriched pathways related to signal processing such as SNARE interactions in vesicular transport, writ signaling, TGF-beta signaling, and taurine and hypotaurine metabolism suggest an enhanced nerve sensitivity to prime the stronger tendency to pollen collection. Our data gain a novel insight into membrane proteome and phosphoproteome-driven cerebral regulation of honeybee behaviors, which is potentially useful for further neurobiological investigation in both honeybees and other social insects.