Isolation of a chromosomally engineered durum wheat line carrying the Aegilops ventricosa Pch1 gene for resistance to eyespot
Isolation of a chromosomally engineered durum wheat line carrying the Aegilops ventricosa Pch1 gene for resistance to eyespot
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DOI：
10.1139/gen-44-3-345
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发表时间：
2001-06-01
期刊：
影响因子：
3.1
通讯作者：
Jahier, J
中科院分区：
文献类型：
--
作者：
Huguet-Robert, V;Dedryver, F;Jahier, J
脑室小麦（Aegilops, 2n = 4x = 28, genome (DDMMv)-D-v-M-v-M-v）染色体7D(v)携带抗眼斑基因Pch1。该基因先前已被转移到面包小麦（T. aestivum, 2n = 6x = 42，基因组AABBDD）的7D染色体上。为了(1)通过增加Pch1拷贝数来提高面包小麦的抗性水平，(2)从伊蚊与黑麦杂交的回交后代中选择携带Pch1的减数稳定硬粒系，以获得抗眼斑的小黑麦。脑室和硬脑膜。Creso ph1c （2n = 4x = 28，基因组AABB）。Pch1转移位于7A染色体长臂的远端位置，可能是同源重组所致。在抗性重组系中，发现7A微卫星标记xgwm698与基因渐渗有密切的排斥关系，内肽酶等位基因位于cv的7A染色体上。Creso ph1c丢失了。
The chromosome 7D(v) of Aegilops ventricosa (syn. Triticum ventricosum, 2n = 4x = 28, genome (DDMMv)-D-v-M-v-M-v) carries the gene Pch1 for resistance to eyespot. This gene has previously been transferred to chromosome 7D of bread wheat, T. aestivum (2n = 6x = 42, genome AABBDD). To (1) enhance the level of resistance of bread wheat by increasing the copy number of Pch1, and (2) create eyespot-resistant triticales, meiotically stable Pch1-carrying durum lines were selected from the backcross progenies of a cross between Ae. ventricosa and T. durum cv. Creso ph1c (2n = 4x = 28, genome AABB). The Pch1 transfer, likely resulting from homoeologous recombination, was located at the distal position on the long arm of chromosome 7A. The 7A microsatellite marker Xgwm 698 was found closely linked in repulsion to the introgression in the resistant recombination lines, and the endopeptidase allele located on chromosome 7A of cv. Creso ph1c was lost.