Differential Analysis of Mycoalexins in Confrontation Zones of Grapevine Fungal Pathogens by Ultrahigh Pressure Liquid Chromatography/Time-of-Flight Mass Spectrometry and Capillary Nuclear Magnetic Resonance
Differential Analysis of Mycoalexins in Confrontation Zones of Grapevine Fungal Pathogens by Ultrahigh Pressure Liquid Chromatography/Time-of-Flight Mass Spectrometry and Capillary Nuclear Magnetic Resonance
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DOI：
10.1021/jf8033539
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发表时间：
2009-02-25
影响因子：
6.1
通讯作者：
Wolfender, Jean-Luc
中科院分区：
文献类型：
--
作者：
Glauser, Gaetan;Gindro, Katia;Wolfender, Jean-Luc
开发了一种原始方法，用于对密闭空间中生长的真菌对抗形成的区域线进行化学和生物学研究。在培养皿中培养了两种与埃斯卡病有关的木材腐烂真菌，Eutypa lata 和 Botryosphaeria obtusa。通过超高压液相色谱结合飞行时间质谱（UHPLC/TOFMS）对纯真菌菌株和对抗区的代谢谱进行差异分析。分离出由对抗诱导的选定代谢物，并通过毛细管核磁共振 (CapNMR) 在亚毫克水平上进行表征。对粗提物和分离的分子进行真菌毒性和植物毒性测定。虽然纯菌株的提取物没有活性，但来自对抗区的提取物却表现出显着的活性。一种非常强烈的诱导化合物，O-甲基蜜肽，可以解释这些毒性。所开发的方法展示了真菌对抗作为生物活性分子的原始来源的用途，并为埃斯卡疾病的研究提供了新的见解。
An original approach was developed for the chemical and biological investigation of zone lines formed by the confrontation of fungi growing in confined spaces. Two wood-decaying fungi involved in esca disease, Eutypa lata and Botryosphaeria obtusa, were grown in Petri dishes. Metabolic profiles of pure fungal strains and confrontation zones were differentially analyzed by ultrahigh pressure liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC/TOFMS). Selected metabolites induced by the confrontation were isolated and characterized by capillary NMR (CapNMR) at the submilligram level. Fungitoxic and phytotoxic assays were applied to the crude extracts and isolated molecules. While the extracts of pure strains were inactive, the extract from confrontation zones exhibited significant activities. A very strongly induced compound, O-methylmellein, may explain these toxic properties. The developed approach demonstrates the use of fungal confrontations as an original source of bioactive molecules and gives new insights into the study of esca disease.