A high-density transcript linkage map of barley derived from a single population
A high-density transcript linkage map of barley derived from a single population
复制标题
DOI：
10.1038/hdy.2009.57
复制
发表时间：
2009-08-01
期刊：
影响因子：
3.8
通讯作者：
Takeda, K.
中科院分区：
文献类型：
--
作者：
Sato, K.;Nankaku, N.;Takeda, K.
利用一个双单倍体作图群体，仅3 ′-末端表达序列标签（EST）和仅基于PCR的分析，构建了大麦高分辨率转录本连锁图。品种“Haruna Nijo”和祖先野生型登录“H602”被用作EST供体和作图群体的杂交亲本。在10366对引物中，7700对引物产生了有用的扩增子，3975对引物（52%）在作图亲本之间显示出多态性。其中，2890个（占总数的28%）是由单核苷酸多态性（1717），切割扩增多态性序列（933）和INDEL（240）定位的。目前的工作涉及大麦基因的估计9%。在所绘制的EST中，2689个（93%）在Affyellow Barley 1 GeneChip中格式化，1039个（36%）可获得全长cDNA序列。与小麦基因索引中的序列相似性最高（93%），与水稻的相似性中等（50%）。映射EST位置和水稻假分子的比较表明两个物种之间的共线区域，这些是特别保守的整个大麦染色体3 H和水稻染色体1。这些定位的基因，加上系统开发的一套遗传资源，将使直接克隆显示简单遗传的基因和确定复杂性状的遗传基础变得更加容易。这些信息将有助于制定大麦物理图谱框架，这是基因组测序所必需的。Heredity（2009）103，110-117; doi：10.1038/hdy.2009.57;在线发表2009年5月20日
A high-resolution transcript linkage map of barley was created using a single doubled haploid (DH) mapping population, only 3'-end expressed sequence tags ( ESTs) and only PCR-based assays. Cultivar 'Haruna Nijo' and an ancestral wild-form accession 'H602' were used as EST donors and crossing parents of the mapping population. Of the 10 366 primer sets developed from a non-redundant set of 3'EST sequences, 7700 sets generated useful amplicons and 3975 (52%) showed polymorphisms between the mapping parents. Of these, 2890 (28% of the total) were mapped by single nucleotide polymorphisms ( 1717), cleaved amplified polymorphic sequence ( 933) and INDELs ( 240). The present work involves an estimated 9% of the genes of barley. Of the mapped ESTs, 2689 (93%) are formatted in the Affymetrix Barley 1 GeneChip and full-length cDNA sequences are available for 1039 (36%). Mapped ESTs show highest similarity with sequences in the wheat gene index (93%) and moderate similarity with rice (50%). Comparison of mapped EST positions and the rice pseudomolecule indicated collinear regions between two species; these are particularly conserved for the entire barley chromosome 3H and rice chromosome 1. These mapped genes, together with a systematically developed set of genetic resources, will make it easier to directly clone genes showing simple inheritance and to determine the genetic basis of complex traits. The information will contribute to the development of a framework for the physical mapping of barley, which is necessary for genome sequencing. Heredity ( 2009) 103, 110-117; doi: 10.1038/hdy.2009.57; published online 20 May 2009