FORMATION OF HIGH-DENSITY LIPOPROTEIN-2-LIKE PARTICLES DURING LIPOLYSIS OF VERY LOW-DENSITY LIPOPROTEINS INVITRO
FORMATION OF HIGH-DENSITY LIPOPROTEIN-2-LIKE PARTICLES DURING LIPOLYSIS OF VERY LOW-DENSITY LIPOPROTEINS INVITRO
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DOI：
10.1073/pnas.75.9.4519
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
EISENBERG, S
中科院分区：
文献类型：
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作者：
PATSCH, JR;GOTTO, AM;EISENBERG, S
本文研究了人血浆极低密度脂蛋白（VLDL）脂解对高密度脂蛋白（HDL）结构和组成的影响。在含有来自人血浆的VLDL（d[密度] < 1.006 μ g/ml）和HDL 3（d = 1.125-1.210 μ g/ml）和从牛乳中纯化的脂蛋白脂肪酶（EC 3.1.1.34）的体外受控系统中进行脂解。极低密度脂蛋白的脂解引起HDL 3的深刻变化。在其脂解过程中从VLDL释放的蛋白质、磷脂和胆固醇被转移到HDL 3颗粒。由于这种体外转移，HDL 3的化学组成和生物物理性质发生了实质性改变。新形成的颗粒表现出浮选速率。**图形 **。6.7和水合密度为1.110 g/ml。它们的化学组成与天然HDL 2非常相似，并且它们的大小略大于前体HDL 3。当HDL 3和后脂解HDL 2进行超离心浮选速度和平衡条件下，都被证明是稳定的颗粒。当外推到体内条件时，这些结果表明血浆中循环VLDL和HDL 2水平之间存在重要的代谢关系。这种关系现在允许一个合理的解释，许多在体内观察，其中VLDL和HDL 2的水平变化。
The effects of lipolysis of human plasma very low density lipoprotein (VLDL) on the structure and composition of high density lipoproteins (HDL) were investigated. Lipolysis was performed in a controlled system in vitro containing VLDL (d[density] < 1.006 g/ml) and HDL3 (d = 1.125-1.210 g/ml) from human plasma and lipoprotein lipase (EC 3.1.1.34) purified from bovine milk. Lipolysis of VLDL caused profound changes in HDL3. Protein, phospholipid and cholesterol liberated from VLDL during its lipolysis were transferred to the HDL3 particles. As a consequence of this in vitro transfer, the chemical composition and biophysical properties of HDL3 were substantially altered. The newly formed particles exhibited a flotation rate .**GRAPHIC**. of 6.7 and a hydrated density of 1.110 g/ml. Their chemical composition closely resembled that of native HDL2, and their size was slightly larger than that of the precursor HDL3. When HDL3 and postlipolysis HDL2 were subjected to ultracentrifugation under flotation velocity and equilibrium conditions, both proved to be stable particles. These results, when extrapolated to in vivo conditions, suggest an important metabolic relationship between the levels of circulating VLDL and HDL2 in plasma. This relationship now permits a reasonable explanation for numerous in vivo observations in which the levels of VLDL and HDL2 change reciprocally.