Quorum-sensing quenching by rhizobacterial volatiles
Quorum-sensing quenching by rhizobacterial volatiles
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DOI：
10.1111/j.1758-2229.2011.00284.x
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发表时间：
2011-12-01
影响因子：
3.3
通讯作者：
Vainstein, Alexander
中科院分区：
文献类型：
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作者：
Chernin, Leonid;Toklikishvili, Natela;Vainstein, Alexander
我们发现，根际菌株荧光假单胞菌B-4117和Serratia alummuthica IC 1270产生的挥发性有机化合物（VOCs）可能作为抑制剂的N-酰基高丝氨酸内酯（阿勒）信号分子介导的细胞通讯群体感应（QS）网络由各种细菌，包括菌株的土壤杆菌，色杆菌，果胶杆菌和假单胞菌。当用菌株B-4117和IC 1270释放的VOCs或用菌株IC 1270产生的主要挥发物二甲基二硫醚（DMDS）处理AHL产生菌时，观察到这种群体淬灭效应。LC-MS/MS分析显示，处理菌株绿针假单胞菌449、铜绿假单胞菌PAO 1或Ps.荧光菌2-79与菌株IC 1270或DMDS释放的VOC的混合物显著降低了这些细菌产生的AHLs的量。挥发性有机化合物产生的Ps。chlororaphis 449能够抑制其自身的QS诱导活性，表明同一菌株中VOC和阿勒分子之间存在负相互作用。定量RT-PCR分析表明，PS. chlororaphis 449与IC 1270、B-4117或449自身细胞释放的VOC或与DMDS的组合导致阿勒合酶基因phzI和csaI的转录显著抑制。因此，沿着AHLs，细菌挥发物可能被认为是另一种类型的信号分子参与根际微生物通讯。
We show that volatile organic compounds (VOCs) produced by rhizospheric strains Pseudomonas fluorescens B-4117 and Serratia plymuthica IC1270 may act as inhibitors of the cellcell communication quorum-sensing (QS) network mediated by N-acyl homoserine lactone (AHL) signal molecules produced by various bacteria, including strains of Agrobacterium, Chromobacterium, Pectobacterium and Pseudomonas. This quorum-quenching effect was observed when AHL-producing bacteria were treated with VOCs emitted by strains B-4117 and IC1270 or with dimethyl disulfide (DMDS), the major volatile produced by strain IC1270. LC-MS/MS analysis revealed that treatment of strains Pseudomonas chlororaphis 449, Pseudomonas aeruginosa PAO1 or Ps. fluorescens 2-79 with VOCs emitted by strain IC1270 or DMDS drastically decreases the amount of AHLs produced by these bacteria. Volatile organic compounds produced by Ps. chlororaphis 449 were able to suppress its own QS-induction activity, suggesting a negative interaction between VOCs and AHL molecules in the same strain. Quantitative RT-PCR analysis showed that treatment of Ps. chlororaphis 449 with VOCs emitted by cells of IC1270, B-4117 or 449 itself, or with DMDS, leads to significant suppression of transcription of AHL synthase genes phzI and csaI. Thus, along with AHLs, bacterial volatiles might be considered another type of signal molecule involved in microbial communication in the rhizosphere.