A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus
A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus
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DOI：
10.15252/embj.201899363
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发表时间：
2019-03-15
期刊：
影响因子：
11.4
通讯作者：
Caldelari, Isabelle
中科院分区：
文献类型：
--
作者：
Bronesky, Delphine;Desgranges, Emma;Caldelari, Isabelle
病原菌必须迅速适应不断变化的环境信号，从而导致代谢重塑。由分解代谢控制蛋白A(CCPA)介导的碳分解代谢抑制被用来表达与首选碳源的利用和代谢有关的基因。在这里，我们已经确定RSAI是CCPA抑制的小的非编码RNA，它被高糖浓度抑制。当葡萄糖被消耗时，RSAI抑制编码葡萄糖摄取渗透酶和FN3K酶的mRNAs的翻译启动，FN3K酶保护蛋白质免受高糖浓度的损害。RsaI还与编码胞外多糖产生的转录抑制因子的iCar mRNA的3‘非翻译区以及摄取葡萄糖-6磷酸或一氧化氮诱导的sRNA结合。此外，RSAI的表达伴随着参与碳分解代谢途径的基因转录减少和参与能量产生、发酵和一氧化氮解毒的基因激活。这种多方面的RNA可以被认为是葡萄糖稀缺和生长受阻时的代谢信号。
Pathogenic bacteria must rapidly adapt to ever-changing environmental signals resulting in metabolism remodeling. The carbon catabolite repression, mediated by the catabolite control protein A (CcpA), is used to express genes involved in utilization and metabolism of the preferred carbon source. Here, we have identified RsaI as a CcpA-repressed small non-coding RNA that is inhibited by high glucose concentrations. When glucose is consumed, RsaI represses translation initiation of mRNAs encoding a permease of glucose uptake and the FN3K enzyme that protects proteins against damage caused by high glucose concentrations. RsaI also binds to the 3 ' untranslated region of icaR mRNA encoding the transcriptional repressor of exopolysaccharide production and to sRNAs induced by the uptake of glucose-6 phosphate or nitric oxide. Furthermore, RsaI expression is accompanied by a decreased transcription of genes involved in carbon catabolism pathway and an activation of genes involved in energy production, fermentation, and nitric oxide detoxification. This multifaceted RNA can be considered as a metabolic signature when glucose becomes scarce and growth is arrested.