Temperature induced changes in the starch components and biosynthetic enzymes of two rice varieties
Temperature induced changes in the starch components and biosynthetic enzymes of two rice varieties
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DOI：
10.1007/s10725-005-7361-6
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发表时间：
2005-05-01
影响因子：
4.2
通讯作者：
Zhang, GP
中科院分区：
文献类型：
--
作者：
Cheng, FM;Zhong, LJ;Zhang, GP
研究了温度对水稻胚乳发育过程中淀粉和直链淀粉积累、支链淀粉精细结构以及与淀粉合成相关的一些酶活性的影响。使用两个直链淀粉浓度（AC，%）不同的早籼稻品种，即嘉935（低AC）和嘉353（高AC）。结果表明，高温对AC和支链淀粉精细结构的影响具有品种依赖性。高温导致嘉935直链淀粉浓度降低，支链淀粉短链（CL<22）比例增加；嘉353则相反。高温还分别降低和增加了嘉935和嘉353中颗粒结合淀粉合酶(GBSS)的活性。这表明直链淀粉/淀粉比率因温度而变化可归因于GBSS活性的变化。此外，两个水稻品种的蔗糖合酶（SuSy）、ADP-葡萄糖焦磷酸化酶（ADPG-Ppase）、可溶性淀粉合酶（SSS）、淀粉支化酶（SBE）、淀粉脱支化酶（SDBE）和淀粉磷酸化酶（SPase）在高温下的活性存在明显差异。嘉935的直链淀粉积累率与GBSS显着正相关，而嘉353则不然。两个品种的直链淀粉积累也与SDBE、SBE、ADPG-Ppase和SuSy活性显着正相关。结果表明，水稻胚乳中直链淀粉与淀粉的比例不仅与GBSS有关，还受到SDBE、SBE、ADPG-Ppase和SuSy活性的影响。
The effects of temperature on starch and amylose accumulation, fine structure of amylopectin and activities of some enzymes related to starch synthesis in developing rice endosperms was examined. Two early indica rice varieties were used, differing in amylose concentration (AC, %), namely Jia 935 (low AC) and Jia 353 (high AC). The results showed that the effects of high temperature on AC and amylopectin fine structure were variety-dependent. High temperature caused a reduction in amylose concentration and an increase in the short chain (CL < 22) proportion of amylopectin for Jia 935; while opposite was true for Jia 353. High temperature also reduced and increased the activity of granule-bound starch synthase (GBSS) in Jia 935 and in Jia 353, respectively. This suggests that a change in the ratio of amylose/starch due to temperature was attributable to a change in GBSS activity. Moreover, obvious differences between the two rice varieties were detected in the activities of sucrose synthase (SuSy), ADP-glucose pyrophosphorylase (ADPG-Ppase), soluble starch synthase (SSS), starch branching enzyme (SBE), starch de-branching enzyme (SDBE) and starch phosphorylase (SPase) to high temperature. Accumulation rate of amylose was significantly and positively correlated with GBSS for Jia 935, but not for Jia 353. Amylose accumulation was also significantly and positively correlated with the activities of SDBE, SBE, ADPG-Ppase and SuSy for both varieties. The results suggest that the ratio of amylose to starch in rice endosperm is not only related to GBSS, but also affected by the activities of SDBE, SBE, ADPG-Ppase and SuSy.