Clinical translation of human microRNA 21 as a potential biomarker for exposure to ionizing radiation
Clinical translation of human microRNA 21 as a potential biomarker for exposure to ionizing radiation
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DOI：
10.1016/j.trsl.2014.01.009
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发表时间：
2014-06-01
影响因子：
7.8
通讯作者：
Zabihi, Ebrahim
中科院分区：
文献类型：
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作者：
Halimi, Mohammad;Parsian, Hadi;Zabihi, Ebrahim
本研究探讨了血清microRNA 21 （miR-21）水平在电离辐射（IR）影响下的改变程度。首先，我们评估了我们的RNA提取效率和血清中microRNA测定的适宜性，然后研究了4例乳腺癌患者的血清miR-21水平，分为术前、术后、放疗开始时、25次放疗后共50 Gy的4个阶段，以及20名健康志愿者。初步分析表明，我们的RNA提取效率和血清中微量核糖核酸（microRNA）测定方法适合于识别暴露于IR的人群。然后分析另一组40例乳腺癌患者放疗前后血清miR-21水平。在我们的大规模分析中，放射治疗前miR-21水平与健康志愿者相当（P = 0.10），放射治疗后miR-21水平显著升高(P < 0.001)-这表明该方法可以以高特异性（75%）和敏感性（80%）区分放射患者和未放射患者。根据这项研究，血清miR-21有可能被用作鉴定电离辐射暴露人群的生物标志物。
This study investigated to what extent the serum microRNA 21 (miR-21) level alters in response to ionizing radiation (IR). Initially, we evaluated the appropriateness of our RNA extraction efficiency and microRNA assay in serum, and then investigated the serum miR-21 level in 4 patients with breast cancer in 4 stages: pre- and postoperation, at the beginning radiotherapy, and after 25 sessions of radiotherapy with a total of 50 Gy irradiation, as well as in 20 healthy volunteers. The initial analysis showed the appropriateness of our RNA extraction efficiency and microRNA assay in serum for identifying people exposed to IR. We then analyzed the serum miR-21 level in another group of 40 patients with breast cancer before and after radiotherapy. During our large-scale analysis, the miR-21 level before radiotherapy was comparable with healthy volunteers (P = 0.10) and increased significantly after radiotherapy (P < 0.001)-an indication that this could discriminate irradiated patients from nonirradiated ones with high specificity (75%) and sensitivity (80%). According to this study, serum miR-21 has the potential to be used as a biomarker for the identification of people exposed to ionizing radiation.