Periplakin, a novel component of cornified envelopes and desmosomes that belongs to the plakin family and forms complexes with envoplakin.
Periplakin, a novel component of cornified envelopes and desmosomes that belongs to the plakin family and forms complexes with envoplakin.
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DOI：
10.1083/jcb.139.7.1835
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发表时间：
1997-12-29
期刊：
影响因子：
--
通讯作者：
Watt FM
中科院分区：
文献类型：
--
作者：
Ruhrberg C;Hajibagheri MA;Parry DA;Watt FM
皮质被膜是一层转氨酶交联蛋白，组装在表皮最外层角质形成细胞的质膜下。我们已经确定了一种蛋白质的cDNA序列，该蛋白质被纳入培养的表皮角质形成细胞的皮质包膜中，该蛋白质的表观分子量为195 kD，由估计大小为6.3 kb的mRNA编码。该蛋白质在角化和非角化复层鳞状上皮和许多其他上皮中表达。在体内和培养的表皮角质形成细胞的终末分化过程中，蛋白质的表达上调。免疫金电子显微镜被用来证明协会的195 kD蛋白与桥粒斑块和角蛋白丝在分化层的表皮。序列分析表明，195-kD蛋白是蛋白质的斑蛋白家族的成员，其中envoplakin，桥斑蛋白，大疱性类天疱疮抗原1，和plectin属于。Envoplakin和195-kD蛋白共免疫沉淀。对它们的杆结构域序列的分析表明，同源二聚体和异源二聚体的形成在能量上是有利的。共聚焦免疫荧光显微镜培养的表皮角质形成细胞显示，envoplakin和195-kD蛋白形成一个网络，从桥粒辐射，我们推测，这两种蛋白质可能提供了一个支架上组装的皮质包膜。我们建议将195 kD蛋白命名为periplakin。
The cornified envelope is a layer of transglutaminase cross-linked protein that is assembled under the plasma membrane of keratinocytes in the outermost layers of the epidermis. We have determined the cDNA sequence of one of the proteins that becomes incorporated into the cornified envelope of cultured epidermal keratinocytes, a protein with an apparent molecular mass of 195 kD that is encoded by a mRNA with an estimated size of 6.3 kb. The protein is expressed in keratinizing and nonkeratinizing stratified squamous epithelia and in a number of other epithelia. Expression of the protein is upregulated during the terminal differentiation of epidermal keratinocytes in vivo and in culture. Immunogold electron microscopy was used to demonstrate an association of the 195-kD protein with the desmosomal plaque and with keratin filaments in the differentiated layers of the epidermis. Sequence analysis showed that the 195-kD protein is a member of the plakin family of proteins, to which envoplakin, desmoplakin, bullous pemphigoid antigen 1, and plectin belong. Envoplakin and the 195-kD protein coimmunoprecipitate. Analysis of their rod domain sequences suggests that the formation of both homodimers and heterodimers would be energetically favorable. Confocal immunofluorescent microscopy of cultured epidermal keratinocytes revealed that envoplakin and the 195-kD protein form a network radiating from desmosomes, and we speculate that the two proteins may provide a scaffolding onto which the cornified envelope is assembled. We propose to name the 195-kD protein periplakin.