LOCALIZATION OF PHOSPHATASE ACTIVITIES AT LEVEL OF ULTRASTRUCTURE
LOCALIZATION OF PHOSPHATASE ACTIVITIES AT LEVEL OF ULTRASTRUCTURE
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DOI：
10.1177/12.2.72
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发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
ESSNER, E
中科院分区：
文献类型：
--
作者：
GOLDFISCHER, S;NOVIKOFF, AB;ESSNER, E
总结了该领域的进展。电子显微照片显示的细胞内定位包括“质膜”：核苷磷酸酶、碱性磷酸酶； “内质网”：核苷二磷酸酶、硫胺素焦磷酸酶，在少数情况下还有葡萄糖 6-磷酸酶、酸性磷酸酶和核苷磷酸酶；“高尔基体”：核苷二磷酸酶、硫胺素焦磷酸酶，在少数情况下还有碱性和酸性磷酸酶；“溶酶体”：酸性磷酸酶（也有酯酶）；“线粒体”：Sabatini 等人最近介绍的各种溶酶体和甲醛-钙作为磷酸酶活性的固定剂进行了比较。
Progress in this field is summarized. The intracellular localizations, illustrated by electron micrographs, include "Plasma Membrane": nucleoside phosphatases, alkaline phosphatase; Endoplasmic Reticulum": nucleoside diphosphatase, thiamine pyrophosphatase, and, in fewer instances, glucose-6-phosphatase, acid phosphatase and nucleoside phosphatase; "Golgi saccules": nucleoside diphosphatase, thiamine pyrophosphatase, and, in a few instances, alkaline and acid phosphatases; "Lysosomes": acid phosphatase (also esterase); "Mitochondria": "ATPase". Varieties of lysosomes are illustrated. Glutaraldehyde, recently introduced by Sabatini et al., and formaldehyde-calcium are compared as fixatives for electron microscopy of phosphatase activities.