Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2.
Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2.
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雄性豚鼠直肠内接种 2 型单纯疱疹病毒后生殖器上皮疾病和免疫力的发展。
DOI：
10.1016/j.virol.2018.10.023
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Milligan,GreggN
中科院分区：
文献类型：
--
作者：
Bourne,Nigel;Banasik,BrianneN;Perry,ClariceL;Miller,AaronL;White,Mellodee;Pyles,RichardB;Milligan,GreggN
大多数生殖器对单纯疱疹病毒2型（HSV-2）的免疫分析都是在女性中进行的，因此对男性生殖器上皮的免疫保护还不完全了解。我们建立了一个由豚鼠直肠内接种引起的雄性生殖器HSV-2感染模型。在会阴和包皮上出现短暂的水泡性病变，同时伴有急性病毒脱落。病毒脱落和复发性生殖器病变后，也检测到建立一个潜伏感染。会阴和包皮RNA分析检测到IFNγ、促炎和调节细胞因子以及参与白细胞迁移和调节的基因的转录物。HSV特异性T细胞检测淋巴和生殖器组织后，决议的主要感染，而病毒特异性抗体分泌细胞仅在淋巴组织中检测到。总之，在豚鼠模型中量化发病机制和局部免疫的能力代表了理解雄性对HSV-2免疫的重要进展
Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue. Taken together, the ability to quantify pathogenesis and local immunity in this guinea pig model represent an important advance towards understanding immunity to HSV-2 in males