Chemoselective ligation techniques: Modern applications of time‐honored chemistry
Chemoselective ligation techniques: Modern applications of time‐honored chemistry
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DOI：
10.1002/bip.21337
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发表时间：
2010
期刊：
影响因子：
2.4
通讯作者：
T. Tiefenbrunn;P. Dawson
中科院分区：
文献类型：
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作者：
T. Tiefenbrunn;P. Dawson
化学选择性连接技术能够在稀水溶液中选择性修饰蛋白质和其他生物分子。重要的是，这些反应发生在生理pH或接近生理pH，并且与生物大分子中常见的复杂官能团阵列相容，包括蛋白质，核苷酸和碳水化合物，允许在未受保护的底物上进行缀合反应。最近，越来越多的具有合成有机化学中的既定效用的反应已被证明在水介质中的生物分子的背景下具有令人惊讶的效用。在这篇综述中，我们强调了几个有前途的反应，可能具有广泛的适用性，在新材料的基础上的生物大分子。© 2010 Wiley Periodicals，Inc. Biopolymers（Pept Sci）91：95-106，2010.
Chemoselective ligation techniques enable the selective modification of proteins and other biomolecules in dilute aqueous solution. Importantly, these reactions occur at or near physiological pH and are compatible with the complex array of functional groups commonly found in biological macromolecules including proteins, nucleotides, and carbohydrates, allowing conjugation reactions to be carried out on unprotected substrates. Recently, a growing number of reactions with established utility in synthetic organic chemistry have been shown to have surprising utility in the context of biological molecules in aqueous media. In this review we highlight several promising reactions that may have widespread applicability in the generation of new materials based on biological macromolecules. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 91:95–106, 2010.