Biomimetic and electrolytic calcium phosphate coatings on titanium alloy: physicochemical characteristics and cell attachment
Biomimetic and electrolytic calcium phosphate coatings on titanium alloy: physicochemical characteristics and cell attachment
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DOI：
10.1016/s0142-9612(03)00559-3
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发表时间：
2004-02-01
期刊：
影响因子：
14
通讯作者：
de Groot, K
中科院分区：
文献类型：
--
作者：
Wang, J;Layrolle, P;de Groot, K
“生物仿生沉积的磷酸八钙和碳酸磷灰石以及电解沉积的碳酸磷灰石被认为是传统等离子喷涂羟基磷灰石的有前途的替代品。本研究比较了它们的理化特性和细胞粘附行为。理化特性包括扫描电镜观察、X射线衍射分析、傅里叶变换红外光谱分析、表面粗糙度、涂层厚度、溶解试验和划痕试验。细胞贴壁实验包括体视显微镜和扫描电镜观察细胞形态，DNA含量测定细胞计数。OCP涂层具有100%的结晶度，约40 μ m厚，由30 μ m的大片状晶体组成，具有最低的表面粗糙度（R-a = 2.33 μ m）。BCA涂层的结晶度为60%，厚度约为30 μ m，由尺寸为1-2 μ m的小晶体组成，具有最高的表面粗糙度（R-a = 4.83 μ m）。ECA涂层具有中间特性，具有78%的结晶度、45 μ m的厚度、5-6 μ m的晶体和3.87 μ m的平均粗糙度。肉眼可见所有涂层在酸性模拟体液（模拟生理溶液-SPS，pH 3.0）中快速且完全溶解，但在中性SPS（pH 7.3）中缓慢且不完全溶解。结果表明，在酸性SPS中，溶解度等温线是决定涂层溶解的主要因素，而在中性SPS中，结晶度和晶粒尺寸等因素也是影响涂层溶解的主要因素。划痕试验结果表明，涂层第一次开裂时的临界载荷（L-c1）与晶粒尺寸及其排列方式密切相关，而涂层完全剥离时的临界载荷（L-c2）与涂层厚度也有关。ECA涂层具有最高值。由于更高的溶解速率和球形外观，BCA涂层在1天和3天时表现出最好的山羊骨髓基质细胞附着，其次是OCP和ECA涂层。(C)2003 Elsevier Ltd.保留所有权利。
`Biomimetically deposited octacalcium phosphate (OCP) and carbonate apatite (BCA) as well as electrolytically deposited carbonate apatite (ECA) were considered as promising alternatives to conventional plasma spraying hydroxyapatite. This study compared their physicochemical characteristics and cell attachment behavior. The physicochemical characteristics included scanning electron microscopy observation, X-ray diffraction analysis, Fourier transform infrared spectroscopy analysis, surface roughness, coating thickness, dissolution test and scratch test. Cell attachment tests included morphology observation with stereomicroscopy and scanning electron microscopy as well as cell number count with DNA content assay. The OCP coating had 100% crystallinity and was about 40 mum thick, composed of large plate-like crystals of 30 mum, with the lowest surface roughness (R-a = 2.33 mum). The BCA coating had 60% crystallinity and was approximately 30 mum in thickness, composed of small crystals of 1-2 mum in size, with the highest surface roughness (R-a = 4.83 mum). The ECA coating had intermediate characteristics, with 78% crystallinity, 45 pm thickness, crystals of 5-6 mum and an average roughness of 3.87 mum. All coatings could be seen by eyes dissolving quickly and completely into acidic Simulated body fluid (simulated physiological solutions-SPS, pH 3.0) but slowly and incompletely into neutral SPS (pH 7.3). It was suggested that the main factor determining coating dissolution in acidic SPS was the solubility isotherm, while some other factors including crystallinity and crystal size joined to determine coating dissolution in neutral SPS. In regard to adhesive strength, results of scratch test showed the critical load at the first crack of coating (L-c1) was tightly related to crystal size as well as their arrangement, while the critical load at the total delamination of coating (L-c2) was also related to the coating thickness. The ECA coating had the highest values. Owing to higher dissolution rate and globular appearance, BCA coating demonstrated the best goat bone marrow stromal cells attachment at 1 day or 3 days, followed by OCP and ECA coating. (C) 2003 Elsevier Ltd. All rights reserved.