Assessment of the Reproducibility of Random Hexapeptide Peptide Library-Based Protein Normalization
Assessment of the Reproducibility of Random Hexapeptide Peptide Library-Based Protein Normalization
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DOI：
10.1021/pr900608z
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Wilkins, John A.
中科院分区：
文献类型：
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作者：
Dwivedi, Ravi C.;Krokhin, Oleg V.;Wilkins, John A.
生物样品中蛋白质的宽动态范围对低丰度蛋白质的检测提出了挑战。最近，组合六肽肽库已被建议作为一种方法，在这样的混合物中的蛋白质的归一化。我们研究了商业六肽配体库的定量和比较血清蛋白质组学分析的重现性。我们还比较了这种技术与基于IgY的亲和耗竭。
The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commercial hexapeptide ligand library for quantitative and comparative serum proteomic analysis. We also compared this technology with IgY-based affinity depletion.