Chemical analysis of acid mucopolysaccharides of mixed salivary tumours.
Chemical analysis of acid mucopolysaccharides of mixed salivary tumours.
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DOI：
10.1038/bjc.1966.56
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发表时间：
1966-09
影响因子：
8.8
通讯作者：
Schorah, C J
中科院分区：
文献类型：
--
作者：
Lovell, D;Briggs, J C;Schorah, C J
材料和方法酸性粘多糖的提取、分离和测定手术切除肿瘤后立即获得肿瘤，并取代表性块进行组织学检查。将用于化学分析的样品细分并用丙酮处理24小时，然后在2天内多次更换由等体积的氯仿和甲醇组成的混合物，并干燥至恒重。干燥组织（约105 mg.）用结晶木瓜蛋白酶（BDH）在650 ℃消化。0.05 M KH 2 PO中。NaOH缓冲液，pH 6-5，含6-0 mg。每10毫升含半胱氨酸-HCl和18 -6毫克EDTA（二钠盐）。缓冲液18小时。通过加入三氯乙酸至终浓度为7 × 5%（w/v），将消化物脱蛋白，离心，用3体积乙酸钠饱和的乙醇从上清液中沉淀多糖，并溶解在蒸馏水中。
MATERIALS AND METHODS Extraction, separation and determination of acid mucopolysaccharides The tumours were obtained immediately following surgical removal and representative blocks taken for histology. The samples for chemical analysis were finely divided and treated with acetone for 24 hours, followed by several changes of a mixture consisting of equal volumes of chloroform and methanol over a period of 2 days, and dried to constant weight. The dried tissues (approxi-mately 5 mg.) were digested with crystalline papain (BDH) at 650 C. in 0.05 M KH2PO.-NaOH buffer, pH 6-5 containing 6-0 mg. cysteine-HCl and18-6 mg.EDTA (disodium salt) per 10 ml. of buffer for 18 hours. The digest was deproteinised by the addition of trichloroacetic acid to a final concentration of 7* 5 per cent (w/v), centrifuged and the polysaccharides precipitated from the supernatant with 3 vol. of ethanol saturated with sodium acetate and dissolved in distilled water.