Frequency of human cytomegalovirus-specific T cells during pregnancy determined by intracellular cytokine staining
Frequency of human cytomegalovirus-specific T cells during pregnancy determined by intracellular cytokine staining
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DOI：
10.1002/jmv.10556
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发表时间：
2003-12-01
影响因子：
12.7
通讯作者：
Tsutsumi, H
中科院分区：
文献类型：
--
作者：
Fujikawa, T;Numazaki, K;Tsutsumi, H
研究了巨细胞病毒(CMV)特异性T细胞免疫功能在初次感染、潜伏感染或再激活感染的孕妇中的特点，并与对照组的非妊娠妇女进行了比较。对46例妊娠妇女和8例非妊娠妇女进行了血清CMV抗体活性和尿液病毒排泄的检测。通过细胞内细胞因子、干扰素-γ和肿瘤坏死因子-α的染色，检测外周血中巨细胞病毒特异性的CD4(+)T细胞的频率。在CMV血清阳性的正常非孕和妊娠妇女中，CMV特异性的CD4(+)T细胞的频率在任何孕期均无变化。然而，与CMV再激活或再感染相关的孕妇的CMV特异性CD4(+)T细胞的频率显著高于CMV血清阳性的正常孕妇和非孕妇。所有这些妇女的婴儿都没有巨细胞病毒传播。孕妇体内的这些巨细胞病毒特异性T细胞反应可能有助于阻断婴儿宫内巨细胞病毒感染。(C)2003年Wiley-Liss，Inc.
The characteristics of cytomegalovirus (CMV)-specific T-cell immunity was investigated in pregnant women with primary, latent, or reactivated CMV infection, and in a comparative group of non-pregnant women. Forty-six pregnant and 8 non-pregnant women were examined based on the presence of serum antibody activity against CMV and viral excretion in urine. The frequency of CMV-specific CD4(+) T cells in peripheral blood lymphocytes was determined by staining for intracellular cytokines, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha. There was no change in the frequencies of CMV-specific CD4(+) T cells in CMV-seropositive normal non-pregnant and pregnant women at any gestation. However, the frequency of CMV-specific CD4(+) T cells in pregnant women associated with CMV reactivation or reinfection was significantly higher than in CMV-seropositive normal pregnant and non-pregnant women. There were no CMV transmissions to the infants of all these women. These CMV-specific T cells responses in pregnant women may contribute some to block the intrauterine CMV infection in their infants. (C) 2003 Wiley-Liss, Inc.